SUN Xiao-xue, KANG Jie, WANG Chang, et al. Optimization of Purification Process for Total Polysaccharides in Stigma Maydis with DEAE Cellulose[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 28-30.
DOI:
SUN Xiao-xue, KANG Jie, WANG Chang, et al. Optimization of Purification Process for Total Polysaccharides in Stigma Maydis with DEAE Cellulose[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 28-30. DOI: 10.13422/j.cnki.syfjx.2014150028.
Optimization of Purification Process for Total Polysaccharides in Stigma Maydis with DEAE Cellulose
Objective: To optimize purification process of total polysaccharides from Stigma Maydis by DEAE cellulose and determine proportion of neutral and acidic polysaccharides. Method: Anthrone sulfuric acid method was adopted to determine the content of total polysaccharides with detection wavelength at 625 nm.Single factor tests were used to investigate effects of sample amount
concentration and dosage of eluant on purifition process of total polysaccharides from Stigma Maydis by DEAE cellulose. Result: The best purification process was as following:sample volume of 0.75 BV
eluted with 10 BV of water and 15 BV of 0.1 mol·L-1 NaCl solution;concentrations of neutral and acidic polysaccharides in total polysaccharides were 30.43% and 58.88%
purities of them increased about 2.5 and 2 times
respectively. Conclusion: Purification of total polysaccharides from Stigma Maydis with DEAE cellulose was suitable and it could separate neutral polysaccharides and acid polysaccharides at the same time.