Quantitative Analysis of Aflatoxin in Sterculiae Lychnophorae Semen by HPLC-FLD after Immunoaffinity Column with Post-column Photochemical Derivatization
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Quantitative Analysis of Aflatoxin in Sterculiae Lychnophorae Semen by HPLC-FLD after Immunoaffinity Column with Post-column Photochemical Derivatization
Chinese Journal of Experimental Traditional Medical FormulaeVol. 20, Issue 15, Pages: 75-78(2014)
SU Jing-hua, ZHANG Chao, ZHONG Shui-sheng, et al. Quantitative Analysis of Aflatoxin in Sterculiae Lychnophorae Semen by HPLC-FLD after Immunoaffinity Column with Post-column Photochemical Derivatization[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 75-78.
DOI:
SU Jing-hua, ZHANG Chao, ZHONG Shui-sheng, et al. Quantitative Analysis of Aflatoxin in Sterculiae Lychnophorae Semen by HPLC-FLD after Immunoaffinity Column with Post-column Photochemical Derivatization[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 75-78. DOI: 10.13422/j.cnki.syfjx.2014150075.
Quantitative Analysis of Aflatoxin in Sterculiae Lychnophorae Semen by HPLC-FLD after Immunoaffinity Column with Post-column Photochemical Derivatization
Objective: To establish a stable method for quantitative analysis of aflatoxin in Sterculiae Lychnophorae Semen by HPLC-FLD after immunoaffinity column with post-column photochemical derivatization. Method: Cloversil C18 column was applied and mobile phase was consisted of methanol and water (47:53) with gradient elution of 1 mL·min-1
column temperature was maintained at 35℃. Result: The detection limits and quantitation limits of aflatoxin were lower than 0.2 μg·L-1
average recoveries were in range of 97.06%-99.93%
and RSDs were below 12%. Besides that
precisions and repetitiveness all achieve the requirement. Conclusion: The method is a simple
accurate and could be used to analyze the content of aflatoxin in Sterculiae Lychnophorae Semen.
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Related Author
Lu LIU
Sheng-min QIU
Xi-xian YANG
Yi-chen HU
Jie ZHOU
Long WANG
Chun-sheng LIU
Guo-dong HUA
Related Institution
Key Laboratory of Coarse Cereal Processing,Ministry of Agriculture and Rural Affairs,Chengdu University
School of Chinese Materia Medica, Beijing University of Chinese Medicine
Dongfang Hospital, Beijing University of Chinese Medicine