Determination of Content of Syringin from Different Fractions in Cortex Daphnes by HPLC

ZHANG Jin; ZHANG Chao

doi:10.13422/j.cnki.syfjx.2014150096

您当前的位置：

首页 >

文章列表页 >

Determination of Content of Syringin from Different Fractions in Cortex Daphnes by HPLC

更新时间：2024-01-04

- Determination of Content of Syringin from Different Fractions in Cortex Daphnes by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 15, Pages: 96-98(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014150096
  CLC： O657.72;R284.1
- Published：2014
- 稿件说明：
移动端阅览
ZHANG Jin, ZHANG Chao. Determination of Content of Syringin from Different Fractions in Cortex Daphnes by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 96-98.
DOI：

ZHANG Jin, ZHANG Chao. Determination of Content of Syringin from Different Fractions in Cortex Daphnes by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 96-98. DOI： 10.13422/j.cnki.syfjx.2014150096.

摘要

目的：建立HPLC测定祖师麻中紫丁香苷含量的方法，并比较祖师麻不同部位中紫丁香苷的含量。方法：采用70%甲醇超声提取制备供试品溶液，选用Kromasil C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-0.1%甲酸水（15：85），检测波长265 nm，流速1.0 mL·min-1，柱温25℃。结果：紫丁香苷在3.64～72.80 mg·L-1有良好的线性关系（Y=22.345X-0.517，r=0.999 7），平均回收率为99.47%，RSD 1.50%。结论：紫丁香苷在叶部含量最低，在芯部有较高的含量，可考虑从祖师麻的根芯和茎芯中分离制备紫丁香苷。

Abstract

Objective: To establish a HPLC method for the determination of the content of syringin and compare the distribution of syringin in the different parts of Cortex Daphnes. Method: 70% methanol and supersound was used to extract syringin. Kromasil C18 (4.6 mm×250 mm

5 μm) column was used to analyze syringin content with acetonitrile-0.1% methanoic acid aqueous solution as mobile phases. Flow rate was 1.0 mL·min-1. Detection wavelength was set at 265 nm. Result: Linearity of each standard was established in the concentration range of 3.64-72.80 mg·L-1 for syringin

with correlation coefficient r=0.999 7.Average recoveries (n=6) of syringin was 99.47% with a RSD of 1.50%. Conclusion: The content of syringin in the leaf segment is the lowest. The elder pith is higher in the content of syringin. So

solution and preparation of syringin from the elder pith of root and stem can be considered.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Comparison of Content of Coumarins from Different Fractions in Cortex Daphnes

Effects of Different Drying Methods on Contents of Active Components in Cortex Daphnes

HPLC Fingerprint of Bletillae Formosanae Rhizoma

HPLC Fingerprint of Different Polarity Fractions of Gardeniae Fructus with Different Fruit Shapes

Determination of Amygdalin in Apricot-kernel

Related Author

ZHANG Chao

WANG Jing-long

LIN Gui-tao

Mei WU

Hong-zhe LI

Yong-mei XUE

Qing-zhi ZHANG

Yi-chen RAN

Related Institution

College of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine

Henan University of Chinese Medicine

Zhongjing Wanxi Pharmaceutical Co. Ltd.

Hubei Institute for Food and Drug Control

Shanxi Renyuantang Pharmaceutical Co.,Ltd.

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰