Effect of Jisuikang on Spinal Cord Tissues and Expression of Nerve Growth Factor after Rat Spinal Cord Injury

PAN Ya-lan; MA Yong; GUO Yang; CHENG Ji-hua; HUANG Gui-cheng

doi:10.13422/j.cnki.syfjx.2014150144

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Effect of Jisuikang on Spinal Cord Tissues and Expression of Nerve Growth Factor after Rat Spinal Cord Injury

更新时间：2024-01-04

- Effect of Jisuikang on Spinal Cord Tissues and Expression of Nerve Growth Factor after Rat Spinal Cord Injury
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 15, Pages: 144-149(2014)
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- DOI：10.13422/j.cnki.syfjx.2014150144
  CLC： R285.5
- Published：2014
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PAN Ya-lan, MA Yong, GUO Yang, et al. Effect of Jisuikang on Spinal Cord Tissues and Expression of Nerve Growth Factor after Rat Spinal Cord Injury[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 144-149.
DOI：

PAN Ya-lan, MA Yong, GUO Yang, et al. Effect of Jisuikang on Spinal Cord Tissues and Expression of Nerve Growth Factor after Rat Spinal Cord Injury[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 144-149. DOI： 10.13422/j.cnki.syfjx.2014150144.

摘要

目的：明确脊髓康对脊髓损伤（SCI）后脊髓组织病理的影响，探讨其对神经生长因子（NGF）蛋白和mRNA表达水平的影响，分析其改善损伤轴突再生微环境的可能作用机制。方法：清洁级SD大鼠156只，改良Allen’s法建立SCI模型，随机分为假手术组、模型组、强的松（0.06 g·kg-1·d-1）组、脊髓康高、中、低剂量组（50， 25， 12.5 g·kg-1·d-1）6组，每组26只。各组大鼠均于麻醉苏醒后30 min开始给予相应药物或生理盐水处理。分别于干预后3，7，14 d处死，取材，HE染色、透射电镜观察脊髓组织结构的变化，免疫组化、荧光定量PCR法检测脊髓损伤区NGF蛋白及mRNA的表达情况。结果：HE染色显示随着干预时间延长，强的松组、脊髓康中剂量组脊髓组织改善较为明显，神经元存活较模型组明显增加，水肿、坏死较轻。透视电镜超微结构评分显示伤后7 d强的松组、脊髓康中剂量组较模型组均明显降低（P<0.05）。免疫组化结果显示，模型组及各治疗组NGF阳性表达高于同时间点假手术组，术后7，14 d强的松组、脊髓康中剂量组NGF相对表达水平稳定，与模型组相比有统计学差异（P<0.01）。荧光定量PCR结果显示，各治疗组NGF mRNA的相对表达量均高于模型组，尤以强的松组、脊髓康中剂量组升高趋势最为明显，14 d脊髓康中剂量组NGF mRNA的表达水平高于强的松组，差异有统计学意义（P<0.01）。结论：强的松、脊髓康均可较好地减轻和延缓SCI后的病理损害程度，阻止不可逆性的变性现象的发生和发展，并促进SCI后损伤局部NGF蛋白及mRNA的表达，且强的松在SCI早期效应较为明显，而脊髓康在中晚期的整体调节优势明显，这可能是脊髓康促进神经功能恢复，改善轴突再生微环境的作用机制之一。

Abstract

Objective: To clarify the effect of Jisuikang on neural functional recovery and explore its probable mechanism to improve the axon regeneration microenvironment on protein and mRNA level of nerve growth factor(NGF). Method: The rat models of spinal cord injury(SCI) were established by modified Allen's method. Twenty six rats were randomly allocated into pseudo surgery group (group A)

other 130 rats were randomly divided into model group (group B)

prednisolone group (group C)

high

middle and low doses Jisuikang group (group D-F) after SCI

26 rats in each group. The rats were intervened 30 min after anesthesia recovery. The spinal cord tissue were collected 3

14 d after SCI.The changes of the spinal cord tissues were observed through the HE staining

transmission electron microscope (TEM). Immunohistochemistry and RT-PCR were applied to test the expression of protein and mRNA of NGF. Result: HE staining:with the intervention time extending

the neuron survival in group C and E was more obvious than group B. The TEM ultrastructure scoring:group C and E reduced 7 d after injury(P<0.05).Immunohistochemistry:the different time points expression of NGF of group B-F was significantly higher than group A

the postoperative expression of group C and E remained a high levels after 7-14 d

compared with group B (P<0.01). RT-PCR:the expression of NGF of group C-F were higher than group B

especially in group C and E. The expression of NGF of group E was obvious higher than other groups after 14 d

compared with group C (P<0.01). Conclusion: Both Jisuikang and prednisolone can reduce and delay the pathological damage

prevent the occurrence and development of irreversible deformation phenomenon

and promote the expression of NGF after SCI. Prednisolone has a more obvious effect early after SCI

while Jisuikang has a lasting effect. Maybe it is one of the factors of Jisuikang to promote neural functional recovery and improve the axon regeneration microenvironment.

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