Objective: To discuss the effects of resveratrol on the growth and cell apoptosis of human hepatoblastoma-derived HepG-2 cell line. Method: The resveratrol was extracted by microwave-assisted method and HepG-2 cells were exposed to resveratrol at the different concentrations. The hepatocellular carcinoma HepG-2 cells in logarithmic phase of growth were randomly divided into drug group and control group

with the concentration of 12.5

25

50

100 μmol·L-1 resveratrol in hepatocellular carcinoma HepG-2 cells. Intervention durations were 24

48

72 h accordingly. The viability of HepG-2 cells was measured by MTT. Morphotology of cell apoptosis was observed by Hoeschst 33342 fluorescence staining and fluorescent. The protein expression of Bcl-2 and Bax was analyzed by western blot. Result: Resveratrol inhibited the growth of cells and caused apoptosis significantly. The suppression was both in a time-and dose-dependent manner. The result of MTT showed that

at 50

100 μmol·L-1 could inhibit the cell proliferation obviously compared with control group(P<0.01). When HepG-2 cells were treated with the concentration of 50

100 μmol·L-1 resveratrol in 48 h

the typical apoptotic body can be observed under the fluorescence microscope.The expression of protein Bcl-2 was down-regulated and the expression of peotein Bax was up-regulated along with increase in resveratrol concentration. Conclusion: Resveratrol can inhibit the proliferation of HepG-2 cells in vitro and induce cell apoptosis.Resveratrol may induce apoptosis via down-regulating Bcl-2 and up-regulating of Bax in HepG-2 cells.