Objective: To optimize ultrasonic wave-enzymatic assisted semi-biotic extraction technology for Zanthoxyli Radix. Method: With yields of total alkaloids

nitidine chloride and dry extract as indexes

orthogonal test was adopted to optimize extraction technology by taking ultrasonic power

extraction time and solvent amount as factors

then extraction time was selected by dynamic process.HPLC was employed to determine the content of nitidine chloride with mobile phase of acetonitrile-water-phosphoric acid-triethylamine (30:70:0.15:0.15) and detection wavelength at 271 nm;UV was adopted to determine the content of total alkaloids at 328 nm. Result: Optimal extraction process of Zanthoxyli Radix was as follows:after pretreated by cellulase-pectinase

extracted thrice by 5 times the amount of sodium hydrogen phosphate-citric acid buffer with pH of 2.0

7.5 and 8.3

extraction time of 18

15

12 min

respectively;ultrasonic power of 250 W;yields of total alkaloids

nitidine chloride and dry extract were 1.20%

0.135% and 14.65% with RSD of 1.75%

1.86% and 1.23%

respectively. Conclusion: Dry extract yield of this optimized process is significantly higher than ethanol reflux process

while extracting rates of nitidine chloride and total alkaloids are equal with or little lower than the later

this study could provide a reference for industrial production of Zanthoxyli Radix.