RAPD Analysis of Germplasm Resource in

JI Bao-yu; PEI Li-xin; CHEN Sui-qing; DONG Cheng-ming; FENG Wei-sheng

doi:10.13422/j.cnki.syfjx.2014160056

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RAPD Analysis of Germplasm Resource in

更新时间：2024-01-04

- RAPD Analysis of Germplasm Resource in
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 16, Pages: 56-59(2014)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2014160056
  CLC： R284.1
- Published：2014
- 稿件说明：
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JI Bao-yu, PEI Li-xin, CHEN Sui-qing, et al. RAPD Analysis of Germplasm Resource in [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 56-59.
DOI：

JI Bao-yu, PEI Li-xin, CHEN Sui-qing, et al. RAPD Analysis of Germplasm Resource in [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 56-59. DOI： 10.13422/j.cnki.syfjx.2014160056.

摘要

目的：建立野葛RAPD分子标记技术并对野葛种质资源遗传背景进行探讨。方法：结合葛根素的测定，应用RAPD技术对11个产地野葛进行遗传多样性分析。结果：葛根素含量在3.26% ~7.10%，8 条引物共扩增出45条带，其中37条呈多态性，发现RAPD多态位点为82.22%，证明在野葛种质资源中存在较丰富的遗传多样性。结论：我国野葛具有明显的遗传分化，这些遗传分化是野葛种质资源筛选的关键。

Abstract

Objective: This study aimed to establish the RAPD molecular marker system and to probe into the genetic background of Pueraria lobata. Method: RAPD analyses of selected plants from eleven various habitats in China were carried out using arbitrary primers

in combination with the chemical investigation of puerarin. Result: The percentage of puerarin ranged from 3.26% to 7.10%. A total of 45 DNA fragments were obtained using 8 primers

37 of which were polymorphic

accounting for 82.22%

which can tell that the samples had a high level of genetic diversity. Conclusion: P. lobata has significant genetic differentiation that further support the prospects for germplasm selection.

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