Objective: The purpose of this study was to develop a RP-HPLC method for simultaneous determination of six ingredients (chlorogenic acid

caffeic acid

galuteolin

forsythoside A

forsythin and quercetin) in Lonicerae Japonicae Flos and Forsythiae Fructus extracts. Method: The chromatographic separation was achieved on a Thermo Scientific ODS-2 HYPERSIL (4.6 mm×200 mm

5 μm) column with gradient elution of acetonitrile and 0.2% (v/v) formic acid aqueous solution at a flow rate of 1.0 mL· min-1. The detection wavelength was set at 280 nm

and the column temperature was set at 30℃. Result: The ranges for linear correlation of chlorogenic acid

caffeic acid

forsythoside A

galuteolin forsythin and quercetin were 40.40-404.0 mg· L-1 (r=0.999 5)

0.2032-2.032 mg· L-1 (r=0.999 9)

30.20-302.0 mg· L-1 (r=0.999 8)

1.012-10.12 mg· L-1 (r=0.999 9)

6.980-69.80 mg· L-1 (r=0.999 9)

and 1.980-19.80 mg· L-1 (r=0.999 9)

respectively. The recoveries of the injection samples were 102.6%

99.6%

100.4%

98.3%

100.8% and 99.7% with RSD of 0.9%

2.0%

1.1%

0.4%

2.1% and 0.9%

respectively. Conclusion: The method is simple and accurate. It can be applied for the quality control of the Lonicerae Japonicae Flos and Forsythiae Fructus extracts.