CUI Xiao-qiu, LIU Yun-gang. Ultrasonic-assisted Extraction and Content Determination of Shikonin from Arnebiae Radix by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 94-97.
DOI:
CUI Xiao-qiu, LIU Yun-gang. Ultrasonic-assisted Extraction and Content Determination of Shikonin from Arnebiae Radix by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 94-97. DOI: 10.13422/j.cnki.syfjx.2014160094.
Ultrasonic-assisted Extraction and Content Determination of Shikonin from Arnebiae Radix by HPLC
Objective: To develop a method for ultrasonic-assisted extraction and high performance liquid chromatography (HPLC) determination of shikonin in the roots of Arnebiae Radix. Method: Shikonin was extracted by ultrasonic-assisted-methyl alcohol method. The HPLC determination condition consisted of Shim-pack CLC-ODS-18 (4.6 mm×250 mm
10 μm) and shikonin was detected at 516 nm wavelength by using methyl alcohol-water(85∶ 15)as the mobile phase
column temperature at room temperature and flow rate 1.0 mL·min-1. Result: Shikonin had a good linearity relation in the range 0.127-1.143 μg (r= 0.999 8)
regressive equation were Y=338 763X-198 347. The average recovery was 99.3%
RSD 1.18% (n=6). In contrast with refluxing process
the extraction efficiency of ultrasonic extraction was 1.29 times. Conclusion: The methods of ultrasonic extraction and HPLC determination were proved to be accurate
reliable and repeatable. It can be used to determine natural shikonin content of Arnebiae Radix easily.