Comparison of Content of Coumarins from Different Fractions in Cortex Daphnes

ZHANG Chao; WANG Jing-long; LIN Gui-tao

doi:10.13422/j.cnki.syfjx.2014160105

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Comparison of Content of Coumarins from Different Fractions in Cortex Daphnes

更新时间：2024-01-04

- Comparison of Content of Coumarins from Different Fractions in Cortex Daphnes
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 16, Pages: 105-108(2014)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2014160105
  CLC： R284.1
- Published：2014
- 稿件说明：
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ZHANG Chao, WANG Jing-long, LIN Gui-tao. Comparison of Content of Coumarins from Different Fractions in Cortex Daphnes[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 105-108.
DOI：

ZHANG Chao, WANG Jing-long, LIN Gui-tao. Comparison of Content of Coumarins from Different Fractions in Cortex Daphnes[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(16): 105-108. DOI： 10.13422/j.cnki.syfjx.2014160105.

摘要

目的：研究祖师麻不同部位的香豆素类成分分布。方法：采用HPLC测定不同部位中祖师麻甲素和伞形花内酯的含量，色谱条件：Kromasil C18 色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-0.1% 甲酸溶液（15：85），检测波长326 nm，流速1.0 mL·min-1；采用UV测定不同部位中总香豆素的含量。结果：根皮和老茎皮中祖师麻甲素含量较高，叶中伞形花内酯含量最高，皮部祖师麻甲素和伞形花内酯的含量均高于芯部，总香豆素主要分布在皮部和叶部，其含量是芯部的4~7倍。结论：祖师麻的根皮和老茎皮既是质量较优的部位，又是采收时容易分离获取的部位，祖师麻叶部的药用开发需更深入研究。

Abstract

Objective: This study aimed at investigating the distribution of coumarins in the different parts of Cortex Daphnes. Method: The contents of daphnetin and skimmetin in the different parts of Cortex Daphnes were determined by HPLC. The contents of total coumarins in the different parts of Cortex Daphnes were determined by UV. Result: The content of daphnetin in root bark and stem bark was higher than other parts. The content of skimmetin in leaf was the highest. Both daphnetin and skimmetin in bark had higher content than in elder pith. The total coumarins were mainly distributed in bark and leaf

whose content is 4-7 times that of the elder pith. Conclusion: The root bark and stem bark in Cortex Daphnes are not only the medicinal parts in higher quality

but also the parts which are easy to peel and obtain. The leaf of Cortex Daphnes can be developed and utilized in medicine by further research.

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ZHANG Chao

LIN Gui-tao

Related Institution

School of Pharmacy,Shandong University of Traditional Chinese Medicine,Ji’nan

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