Objective: To investigate small intestine absorption characteristics in rats of glucoraphenin in Raphani Semen extract. Method: Taking in situ rat single-pass intestinal perfusion model

effects of different drug mass concentrations (0.02

0.05

0.1 g· mL-1) on intestinal absorption characteristics of glucoraphenin was investigated

and calculated their absorption rate constant (Ka) and apparent absorption coefficient (Papp).HPLC was employed to determine the content of glucoraphenin with mobile phase of 0.1% phosphoric acid solution-acetonitrile (87:13) and detectopm wavelength at 225 nm. Result: Glucoraphenin had the best stability in K-R test solution with pH of 5.12.In Raphani Semen extract with different concentrations

Ka of glucoraphenin in small intestine of rats were (7.750±1.744)×10-3

(7.204±1.045)×10-3

(7.148±0.837)×10-3 min-1

Papp were (1.519±0.507)×10-3

(1.504±0.617)×10-3

(1.592±0.345)×10-3 cm· min-1

these data had no significant changes. Conclusion: Glucoraphenin was easy-to-absorped component

its absorption mechanism in small intestine of rats was passive diffusion.