Objective: To reveal the therapeutic effects of water-solubility alkaloid after using of Aconitum carmichaelii on heart-failure cell model by studying its influence on myocardial cells' membrane ATP enzyme and relative ions. Method: Culture the myocardial cells

gotten from the neonatal rats

for 5 days until the cells became mature

then nembutal (0.8%) was used to establish the heart-failure cell model for 5 min administrate them with 3 concentrations of A. carmichaelii water-solubility alkaloid (0.01

0.02

0.04 g· L-1) and deslanoside injection (4 mL·L-1) determine the vitality of the myocardial cells' membrane ATP enzyme and the concentration of relative ions 90 min later. Result: Compared with the blank control group

in the model group the survival rates for cells were restrained

the concentrate of Na+

Mg2+ and the activity of Ca2+-ATP enzyme

Ca2+-Mg2+-ATP enzyme was decreased. On the contrary

the concentrate of K+

Ca2+ and the activity of Na+-K+-ATP enzyme was increased. Deslanoside injection (4 mL·L-1) and A. carmichaelii water-solubility alkaloid (1.25×10-3

2.50×10-3

5.00×10-3g·kg-1) could increase the the survival rates of heart-failure cells and the concentrate of Na+

Mg2+

and also enhance the activity of Ca2+-ATP enzyme

Ca2+-Mg2+-ATP enzyme. Conversely

all the administrated groups decreased the concentrate of K+

Ca2+ in the myocardial cells and inhibited the activity of Na+-K+-ATP enzyme. Conclusion: Water-solubility alkaloid of A. carmichaelii has the therapeutic effect on heart-failure cells.