Optimization of Extraction Process of Hirudo Zhusan by Anti-thrombin Titration

DENG Guo-tong; LIANG Shao-yu; WU Yan-mei; SONG Guo-qiang; SHEN Zhi-bin

doi:10.13422/j.cnki.syfjx.2014170010

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Optimization of Extraction Process of Hirudo Zhusan by Anti-thrombin Titration

更新时间：2024-01-04

- Optimization of Extraction Process of Hirudo Zhusan by Anti-thrombin Titration
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 17, Pages: 10-13(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014170010
  CLC： R284.2
- Published：2014
- 稿件说明：
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DENG Guo-tong, LIANG Shao-yu, WU Yan-mei, et al. Optimization of Extraction Process of Hirudo Zhusan by Anti-thrombin Titration[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 10-13.
DOI：

DENG Guo-tong, LIANG Shao-yu, WU Yan-mei, et al. Optimization of Extraction Process of Hirudo Zhusan by Anti-thrombin Titration[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 10-13. DOI： 10.13422/j.cnki.syfjx.2014170010.

摘要

目的：优选水蛭煮散的提取工艺。方法：利用抗凝血酶滴定法测定水蛭中抗凝血酶活性物质含量。以粉碎度为指标，筛选粉碎设备，考察水分对水蛭煮散粉体的影响；以浸膏得率和抗凝血酶活性物质转移率为评价指标，通过单因素试验考察粉碎度、浸泡时间、加水量、煎煮次数、煎煮温度及时间对提取工艺的影响，并与传统饮片进行比较。结果：水蛭煮散的最佳提取工艺为取水蛭最粗粉，加20倍量水浸泡10 min，于100 ℃煎煮提取3次，每次20 min；抗凝血酶活性物质转移率达93.31%，高于传统饮片的52.44%。结论：优选的提取工艺稳定可行，水蛭煮散具有效率高、时间短、能耗低、环保等优点，为水蛭煮散的工业化生产和新药开发提供参考。

Abstract

Objective: To optimize extraction technology of Hirudo Zhusan. Method: The content of antithrombin activity substance was determined by anti-thrombin titration.Taking grinding degree as index to screen smash equipment

then effect of water content on powder of Hirudo Zhusan was investigated;with extract yield and transfer rate of antithrombin activity substance as indexes

single factor tests were adopted to optimize extraction process by taking soaking time

the amount of water

decoction time and so on as factors

then compared with traditional pieces of Hirudo. Result: Optimum extraction parameters was as follows:soaked 10 min with 20 times the amount of water

decocted thrice at 100 ℃ for 20 min each time;transfer rate of antithrombin activity substance could reach as highly as 93.31%

which was significantly higher than traditional pieces of 52.44%. Conclusion: This optimized process was stable and feasible

Hirudo Zhusan had advantages of high efficiency

short time

low energy consumption

environmental protection and so on

it could provide an insight for industrial production and new drug development of Hirudo Zhusan.

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