Effects of Different Processing Methods on Concents of Oligosaccharides and Monotropein in Morindae Officinalis Radix

JING Hai-yi; SHI Ji; CUI Ni; JIA Tian-zhu

doi:10.13422/j.cnki.syfjx.2014170020

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Effects of Different Processing Methods on Concents of Oligosaccharides and Monotropein in Morindae Officinalis Radix

更新时间：2024-01-04

- Effects of Different Processing Methods on Concents of Oligosaccharides and Monotropein in Morindae Officinalis Radix
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 17, Pages: 20-23(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014170020
  CLC： R283;R284.2
- Published：2014
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JING Hai-yi, SHI Ji, CUI Ni, et al. Effects of Different Processing Methods on Concents of Oligosaccharides and Monotropein in Morindae Officinalis Radix[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 20-23.
DOI：

JING Hai-yi, SHI Ji, CUI Ni, et al. Effects of Different Processing Methods on Concents of Oligosaccharides and Monotropein in Morindae Officinalis Radix[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 20-23. DOI： 10.13422/j.cnki.syfjx.2014170020.

摘要

目的：考察不同炮制工艺对巴戟天中寡糖类成分和水晶兰苷含量的影响。方法：采用不同方法制备巴戟天炮制品，比较不同炮制品中寡糖类成分和水晶兰苷的含量。利用HPLC-CAD测定寡糖类成分含量，Shodex Asahipak NH2色谱柱，流动相乙腈（A）-水（B）梯度洗脱（0~15 min，86%~81%A；15~20 min，81%~75%A；20~35 min，75%~70%A；35~50 min，70%~58%A；50~60 min，58%~40%A；60~65 min，40%~20%A）；运用HPLC-DAD测定水晶兰苷含量，Venusil MP C18色谱柱，检测波长235 nm，流动相甲醇-0.4%磷酸溶液梯度洗脱（5：95~28.8：71.2，15 min）。结果：巴戟天不同炮制品中寡糖类成分总质量分数顺序为盐巴戟天 >巴戟肉 >制巴戟天 >生巴戟天；水晶兰苷含量排序为盐巴戟天 >生巴戟天 >制巴戟天 >巴戟肉。结论：巴戟天不同炮制品中寡糖类成分和水晶兰苷的含量差异较大，为巴戟天的饮片质量控制及炮制机制解析提供参考。

Abstract

Objective: To investigate effects of different processing methods on concents of oligosaccharides and monotropein in Morindae Officinalis Radix. Method: Processed products of Morindae Officinalis Radix were prepared by different methods

contents of oligosaccharides and monotropein in different processed products were compared.The content of oligosaccharides was determined by HPLC-CAD with Shodex Asahipak NH2 column

mobile phase of acetonitrile(A)-water(B) for gradient elution (0-15 min

86%-81%A;15-20 min

81%-75%A;20-35 min

75%-70%A;35-50 min

70%-58%A;50-60 min

58%-40%A;60-65 min

40%-20%A);the content of monotropein was determined by HPLC-DAD with Venusil MP C18 column

mobile phase of methanol-0.4% phosphoric acid for gradient elution (5:95-28.8:71.2

15 min) and detection wavelength at 235 nm. Result: The content of oligosaccharides was in the order of Morindae Officinalis Radix steamed by salt >Morindae Officinalis Radix without the wood >Morindae Officinalis Radix processed with Glycyrrhizae Radix et Rhizoma >crude Morindae Officinalis Radix;while according to the content of monotropein was Morindae Officinalis Radix steamed by salt >crude Morindae Officinalis Radix >Morindae Officinalis Radix processed with Glycyrrhizae Radix et Rhizoma >Morindae Officinalis Radix without the wood. Conclusion: Processing could greatly change contents of oligosaccharides and monotropein in Morindae Officinalis Radix

which provided a reference for pieces quality control and processing mechanisms analysis of Morindae Officinalis Radix.

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