WANG Li-hong, TAN Feng-tian, ZHAO Hong, et al. Optimization of Extraction Process for Chlorogenic Acid from by Box-Behnken Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 24-26.
DOI:
WANG Li-hong, TAN Feng-tian, ZHAO Hong, et al. Optimization of Extraction Process for Chlorogenic Acid from by Box-Behnken Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 24-26. DOI: 10.13422/j.cnki.syfjx.2014170024.
Optimization of Extraction Process for Chlorogenic Acid from by Box-Behnken Response Surface Methodology
Objective: To optimize extraction process of chlorogenic acid from Lepisorus ussuriensis. Method: On the basis of single factor tests
taking material-liquid ratio
ethanol concentration and extracting time as independent variables
yield of chlorogenic acid in response to a value
according to Box-Benhnken experiment design principle
extraction process of chlorogenic acid from L. ussuriensis was optimized by response surface analysis. Result: Optimum extraction process was as following:extracted twice with 68 times the amount of 75% ethanol for 41 min per time;extracting amount of chlorogenic acid was 8.26 mg·g-1
which was close to the theoretical prediction of 8.47 mg·g-1. Conclusion: Compared with traditional processes
alcohol extraction process had characteristics
such as high extraction efficiency
less energy consumption
low cost
et al.This optimized process was stable and feasible for providing a reference for industrial production of L. ussuriensis.
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Related Author
WANG Ping
LIANG Shaojia
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YI Hong
LI Chun
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Related Institution
Institute of Traditional Chinese Medicine(TCM),Shanghai University of TCM
Yanling DU Renkang Biotechnology Co. Ltd.
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences
Henan University of Chinese Medicine
Engineering Technology Research Center for Comprehensive Development and Utilization of Authentic Medicinal Materials in Henan Province, Henan University of Chinese Medicine