Objective: To optimize purification technology of total polyphenols from Juglandis Semen kernel pellicles by macroporous resin. Method: With Juglandis Semen kernel pellicles as raw material

total polyphenols was extracted by ethanol refluxing method

static adsorption-elution test for total polyphenols were carried out to select proper macroporous resin

single factor tests were adopted to optimize purification process by taking the concentration of sample solution

adsorption flow rate

eluent dosage and so on as factors. Result: X-5 macroporous resin showed the best separation efficiency by comparing with other resins

its optimum purification technology was as follows:the concentration of sample solution 3.0 g·L-1 with pH of 5.0

adsorption rate of 3 BV·h-1

sample volume of 9.5 BV

eluted with 2 BV of 70% ethanol

collected eluent;Purity of total polyphenol was enhanced from 64.52% to 79.64% with transfer rate of 89.83% in extract. Conclusion: This optimized process was stable and feasible

X-5 resin was suitable for initial purification of total polyphenols from Juglandis Semen kernel pellicles.