Determination of Isoscutellarein in Different Parts of  in Different Harvest Time by HPLC

CAO Guang-shang; YANG Pei-min; WANG Xin-feng; LI Fang; GAO Peng

doi:10.13422/j.cnki.syfjx.2014170049

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Determination of Isoscutellarein in Different Parts of in Different Harvest Time by HPLC

更新时间：2024-01-04

- Determination of Isoscutellarein in Different Parts of in Different Harvest Time by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 17, Pages: 49-51(2014)
- 作者机构：
- 作者简介：
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- DOI：10.13422/j.cnki.syfjx.2014170049
  CLC： O657.72;R284.2
- Published：2014
- 稿件说明：
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CAO Guang-shang, YANG Pei-min, WANG Xin-feng, et al. Determination of Isoscutellarein in Different Parts of in Different Harvest Time by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 49-51.
DOI：

CAO Guang-shang, YANG Pei-min, WANG Xin-feng, et al. Determination of Isoscutellarein in Different Parts of in Different Harvest Time by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 49-51. DOI： 10.13422/j.cnki.syfjx.2014170049.

摘要

目的：建立高效液相色谱测定不同采收期白花蛇舌草茎、叶中异高山黄芩素含量的方法。方法：采用Kromasil-C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-0.1%磷酸（44：56），检测波长280 nm，柱温35 ℃。结果：异高山黄芩素进样量在0.145~1.595 μg线性关系良好（r=1）。异高山黄芩素的平均加样回收率为 99.97%，RSD 0.95%。8~9月份采收的白花蛇舌草中异高山黄芩素含量最高，叶中含量是茎中含量的3~4倍。结论：该测定方法简便、准确、可靠，可用于白花蛇舌草的质量控制，并为白花蛇舌草合理采收提供理论依据。

Abstract

Objective: The study was aimed to develop a HPLC method for the determination of a new active component isoscutellarein in Hedyotidis diffusa. Method: The column was Kromasil-C18(4.6 mm×250 mm

5 μm). The acetonitrile-0.1% phosphoric acid(44:56) was used as mobile phase

with UV detection was at 280 nm

the detector drift tube and column temperature at 35 ℃. Result: The sample showed good linear relationship with sample volume in the range of 0.145-1.595 μg for isoscutellarein(r=1).The average recovery of isoscutellarein was 99.97%

with a RSD value of 0.95%. The content of isoscutellarein in leaf collected in August-September was the highest and was three to four times that of the stem. Conclusion: The method is simple

accurate

replicable

and can be used for the quality control of isoscutellarein in H. diffusa.The study also provides a theoretical basis for reasonable harvest time of H. diffusa.

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