Simultaneous Determination of Five Components in Uyghur Drug  and by UPLC

doi:10.13422/j.cnki.syfjx.2014170065

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Simultaneous Determination of Five Components in Uyghur Drug and by UPLC

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- Simultaneous Determination of Five Components in Uyghur Drug and by UPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 17, Pages: 65-68(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014170065
  CLC： R29;O657.7
- Published：2014
- 稿件说明：
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Simultaneous Determination of Five Components in Uyghur Drug and by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 65-68.
DOI：

Simultaneous Determination of Five Components in Uyghur Drug and by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 65-68. DOI： 10.13422/j.cnki.syfjx.2014170065.

摘要

目的：建立同时测定维药毛菊苣和菊苣中5种化学成分含量的UPLC分析方法。方法：采用UPLC，Hypersil BDS C18柱（4.6 mm×250 mm，5 μm），乙腈-0.4%磷酸溶液为流动相梯度洗脱，流速1.0 mL·min-1，柱温30 ℃，检测波长254 nm，进样量10 μL。结果：秦皮甲素、秦皮乙素、山莴苣素、菊苣酸、山莴苣苦素分别在0.025~0.225，0.01~0.05，0.2~1.0，0.1~0.5，0.24~1.20 μg线性关系良好，相关系数分别为0.999 3，0.999 9，0.999 8，0.999 9，0.999 9，平均加样回收率分别为99.37%，98.66%，99.63%，100.05%，99.97%。结论：《中国药典》收载两种菊苣的各成分含量差异很大，毛菊苣以山莴苣苦素、山莴苣素和秦皮乙素含量较高，菊苣以菊苣酸含量高，药材的质量控制和评价值得进一步研究。

Abstract

Objective: This study was conduct to establish an UPLC method for the simultaneous determination of five components aesculin

esculetin

lactucin

cichoric acid and lactucopicrin in Uyghur drug Cichorium glandulosum and C. intybus. Method: Hypersil BDS C18 (4.6 mm×250 mm

5 μm) was used as the stationary phase. The mobile phase was composed of acetonitrile and 0.4% phosphoric acid aqueous with a gradient elution. The flow rate was 1.0 mL·min-1. Column temperature was 30 ℃. The detection wavelength was 254 nm. Result: All calibration curves showed good linear regression (r>0.999 3) within test ranges. The validation data indicated excellent precision and good recovery. Conclusion: The method was successfully applied to quantify five components in C. glandulosum and C. intybus

which could be used as comprehensive quality evaluation of Cichorium.

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