Optimization of Protein Extraction Technology of Gekko Swinhoanis

BAO Hua-yin

doi:10.13422/j.cnki.syfjx.2014170077

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Optimization of Protein Extraction Technology of Gekko Swinhoanis

更新时间：2024-01-04

- Optimization of Protein Extraction Technology of Gekko Swinhoanis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 17, Pages: 77-80(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014170077
  CLC： R284.2
- Published：2014
- 稿件说明：
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BAO Hua-yin. Optimization of Protein Extraction Technology of Gekko Swinhoanis[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 77-80.
DOI：

BAO Hua-yin. Optimization of Protein Extraction Technology of Gekko Swinhoanis[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 77-80. DOI： 10.13422/j.cnki.syfjx.2014170077.

摘要

目的：优化建立壁虎药材蛋白质的提取工艺。方法：通过比较超声和回流两种提取方式对壁虎药材蛋白质提取率的影响，筛选适合的提取方式。分别对pH、料液比、提取温度、提取时间4种影响因素进行单因素考察，并采用L9（34）正交试验设计，以蛋白提取率为指标，优选建立壁虎药材蛋白质的提取工艺。结果：超声提取的蛋白质含量显著高于回流提取，建立了壁虎药材蛋白质的最佳提取工艺：pH 12，料液比1：20，提取温度50 ℃，超声提取时间80 min。结论：该研究建立的蛋白质提取工艺稳定、合理、可行，为壁虎药材的质量评价和生物活性物质的进一步研究提供了实验依据。

Abstract

Objective: The purpose of this study was to optimize the extraction technology of protein in Gekko Swinhoanis. Method: The proper extraction method was selected by comparing the effect of ultrasonic and reflux extraction on protein extraction rate of Gekko Swinhoanis. Then single factor analysis was done for four influencing factors

such as pH

ration of liquid to material

temperature and time. The optimized extraction technology was investigated using orthogonal test with the content of protein as the index for the first time. Result: The protein content by ultrasonic extraction was significantly higher than that by reflux extraction. The best extraction condition of protein in Gekko Swinhoanis was proposed

that's the powder of Gekko Swinhoanis was extracted for 80 minutes with 20 times amount of water (pH 12) by ultrasonic. Conclusion: The optimum extraction condition was stable

reliable and feasible

and could provide a experimental basis for formulating the quality standards and further study of bioactive substances of traditional Chinese medicine Gekko Swinhoanis.

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Related Institution

Natural History Museum of China

Beijing Key Laboratory of Chinese Materia Pharmacology，Institute of Basic Medical Sciences， Xiyuan Hospital，China Academy of Chinese Medical Sciences

Beijing University of Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

School of Chinese Materia Medica， Beijing University of Chinese Medicine

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