Silmultaneous Determination of Paeniflorin, Ferulic Acid and Hyperoside in Tiaojing Huoxue Pills by HPLC

XIE Yuan; TU Yao-sheng; SHI Zhi-qi; ZHOU Rong

doi:10.13422/j.cnki.syfjx.2014170084

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Silmultaneous Determination of Paeniflorin, Ferulic Acid and Hyperoside in Tiaojing Huoxue Pills by HPLC

更新时间：2024-01-04

- Silmultaneous Determination of Paeniflorin, Ferulic Acid and Hyperoside in Tiaojing Huoxue Pills by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 17, Pages: 84-87(2014)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2014170084
  CLC： O657.72;R286.0
- Published：2014
- 稿件说明：
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XIE Yuan, TU Yao-sheng, SHI Zhi-qi, et al. Silmultaneous Determination of Paeniflorin, Ferulic Acid and Hyperoside in Tiaojing Huoxue Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 84-87.
DOI：

XIE Yuan, TU Yao-sheng, SHI Zhi-qi, et al. Silmultaneous Determination of Paeniflorin, Ferulic Acid and Hyperoside in Tiaojing Huoxue Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 84-87. DOI： 10.13422/j.cnki.syfjx.2014170084.

摘要

目的：采用HPLC同时测定调经活血片中芍药苷、阿魏酸、金丝桃苷的含量。方法：采用Elipse-C18色谱柱（4.6 mm×150 mm，5 μm），流动相乙腈-0.1%磷酸（14：86），流速 1.00 mL·min-1，柱温25 ℃，检测器DAD，检测波长230 nm（芍药苷），316 nm（阿魏酸），360 nm（金丝桃苷）。结果：芍药苷在160.0~1280.0 μg与峰面积呈良好的线性关系（r=0.999 6），平均回收率为98.38%，RSD1.51%；阿魏酸在67.5~540.0 μg与峰面积呈良好的线性关系（r=0.999 3），平均回收率为98.33%，RSD1.21%；金丝桃苷在17.2~137.6 μg与峰面积呈良好的线性关系（r=0.999 8），平均回收率98.71%，RSD1.27%。结论：该方法简便、灵敏、准确、重复性好，可用于调经活血片的质量控制。

Abstract

Objective: The study was aimed to determine the contents of paeniflorin

ferulic acid and hyperoside in Tiaojing Huoxue pills by HPLC. Method: The chromatographic column was Elipse-C18(4.6 mm×150 mm

5 μm) with acetonitrile-0.1% phosphoric acid (14:86) as mobile phase.The flow rate was fixed at 1.0 mL·min-1

the column temperature was maintained 25 ℃ and the detector was DAD.The detection wavelengths were set at 230 nm

316 nm for paeniflorin

ferulic acid and hyperoside

respectively

the detection wavelength of hyperoside was set at 360nm. Result: There is good linear relationship within the range of 160.0-1 280.0 μg for paeniflorin

67.5-540.0 μg for ferulic acid

and 17.2-137.6 μg for hyperoside. The average revcoveries of paeniflorin

ferulic acid and hyperoside were 98.38%(RSD of 1.51%)

98.33%(RSD of 1.21%) and 98.71%(RSD of 1.27%) respectively. Conclusion: This method is simple

accurate and reproducible.It can be used for the quality control of Tiaojing Huoxue pills.

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