Simultaneous Determination of Paeoniflorin, Liquiritin and Cinnamic Acid in Yangyin Qingfei Granules by RP-HPLC

ZHANG Mi-mi; QI Guang-cai; ZHANG Yue-cheng; LIU A-jing; LIU Zhen-ye

doi:10.13422/j.cnki.syfjx.2014170088

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Simultaneous Determination of Paeoniflorin, Liquiritin and Cinnamic Acid in Yangyin Qingfei Granules by RP-HPLC

更新时间：2024-01-04

- Simultaneous Determination of Paeoniflorin, Liquiritin and Cinnamic Acid in Yangyin Qingfei Granules by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 17, Pages: 88-90(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014170088
  CLC： O657.72;R286.0
- Published：2014
- 稿件说明：
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ZHANG Mi-mi, QI Guang-cai, ZHANG Yue-cheng, et al. Simultaneous Determination of Paeoniflorin, Liquiritin and Cinnamic Acid in Yangyin Qingfei Granules by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 88-90.
DOI：

ZHANG Mi-mi, QI Guang-cai, ZHANG Yue-cheng, et al. Simultaneous Determination of Paeoniflorin, Liquiritin and Cinnamic Acid in Yangyin Qingfei Granules by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(17): 88-90. DOI： 10.13422/j.cnki.syfjx.2014170088.

摘要

目的：建立以高效液相色谱法同时测定养阴清肺颗粒中芍药苷、甘草苷和肉桂酸含量的方法。方法：采用双波长切换-高效液相色谱法对养阴清肺颗粒中芍药苷、甘草苷和肉桂酸进行定量分析。Agilent ZORBAX SB-C18 色谱柱（4.6 mm×250 mm，5 μm），流动相0.2%磷酸水-乙腈梯度洗脱，检测波长276，230 nm，柱温28 ℃，流速1.0 mL·min-1。结果：芍药苷、甘草苷和肉桂酸分别在0.236 8~4.736 mg·L-1（r=0.999 9），0.170 8~3.416 mg·L-1（r=0.999 9），0.035 2~0.704 mg·L-1（r=0.999 9）线性关系良好；平均回收率分别为99.74%，98.82%，98.40%。RSD分别为0.78%，0.97%，1.04%。结论：该方法简便、快速、准确，可用于养阴清肺颗粒的质量控制。

Abstract

Objective: The study was performed to establish a HPLC method for simultaneous determination of paeoniflorin

liquiritin and cinnamic acid in Yangyin Qingfei granules. Method: Paeoniflorin

liquiritin and cinnamic acid were determined simultaneously by HPLC on a ZORBAX SB-C18 column with the mobile phase composed of acetonitrile and 0.2% phosphoric acid in gradient elution. Detection wavelength were set at 276 nm and 230 nm.The column temperature was maintained at 28 ℃. The flow rate was fixed at 1.0 mL·min-1. Result: The calibration curves of paeoniflorin

liquiritin and cinnamic acid showed good linearity within 0.2368-4.736 mg·L-1 (r=0.999 9)

0.170 8-3.416 mg·L-1 (r=0.999 9)

0.035 2-0.70 4 mg·L-1 (r=0.999 9)

respectively. The average recoveries of those were 99.74%

98.82% and 98.40%

respectively. RSDs were 0.78%

0.97% and 1.04%

respectively. Conclusion: The developed HPLC method is fast

simple and accurate and can be used for quality control of Granules of Yangyin Qingfei.

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