Objective: To investigate gastrointestinal absorption of total flavonoids from Apocyni Veneti Folium in rats. Method: Taking rats in situ perfusion model

absorptivity of hyperin and isoquercitrin in gastrointestinal was counted through detecting contents of index components before and after perfused total flavonoids from Apocyni Veneti Folium by HPLC

mobile phase was acetonitrile-0.05% phosphoric acid (18.5:81.5) at a flow rate of 0.90 mL·min-1 and detection wavelength at 360 nm. Result: A verage absorptivity in stomach of hyperin and isoquercitrin were 36.92% and 36.49%

while in intetines were 47.7% and 48.25%

respectively. Conclusion: This study indicated that hyperin and isoquercitrin had good absorptivity in gastronintestinal department

but absorption of the two ingredients in intestines was better than these in stomach

it could provide scientific basis for dosage form design and clinical rational drug of total flavonoids from Apocyni Veneti Folium.