PANG Yu-xin, YUAN Lei, WANG Zhong-yang, et al. Antioxidant Activities and Anti-tyrosinase of Extracts from Different Parts of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 4-8.
DOI:
PANG Yu-xin, YUAN Lei, WANG Zhong-yang, et al. Antioxidant Activities and Anti-tyrosinase of Extracts from Different Parts of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 4-8. DOI: 10.13422/j.cnki.syfjx.2014180004.
Antioxidant Activities and Anti-tyrosinase of Extracts from Different Parts of
Objective: To investigate anti-tyrosinase and in vitro antioxidant activities of crude extracts from different parts of Blumea balsamifera
in order to screen active ingredients with whitening and antioxidant effects. Method: Functional leaves
tender leaves and immature stems of B. balsamifera were refluxed by aqueous
50% ethanol and 95% ethanol
respectively.Then crude extracts were measured tyrosinase inhibitory by enzymology method
and antioxidant activities were detected by DPPH
ABTS and FRAP methods. Result: DPPH free radical scavenging rate of 95% ethanol extract from functional leaves
tender leaves
immature stems of B. balsamifera was higher than other extracts
which was 95.38%
91.32% and 92.49%
respectively.FRAP
ABTS and anti-tyrosinase experiment's result showed that 50% ethanol and 95% ethanol extracts from functional leaves and immature stems possessed good activity.When concentration of each extracts were 1 500 mg·L-1
scavenging rates of aqueous
50% ethanol and 95% ethanol extracts of functional leaves for ABTS radical were 26.54%
82.48% and 67.46%
data of these three extracts of tender leaves were 34.15%
95.39% and 59.59%
while these data of immature stems were 52.05%
39.35% and 38.77%
respectively.Inhibition rates of aqueous
50% ethanol and 95% ethanol extracts of functional leaves on tyrosinase were 24.24%
32.79%
37.97%;these data of tender leaves were 27.12%
36.53% and 34.19%
while immature stems' were 31.36%
26.22% and 24.00%
respectively. Conclusion: Crude extracts from different parts of B. balsamifera have good antioxidant activities and inhibitory effect on tyrosinase
functional leaves and tender leaves are main active parts.