Extraction and Monosaccharide Composition Analysis of Total Polysaccharides from

MOU Zhen-zhen; WANG Ming-fang; GAO Wen-wen; ZHANG Na; YU Shu-wen

doi:10.13422/j.cnki.syfjx.2014180013

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Extraction and Monosaccharide Composition Analysis of Total Polysaccharides from

更新时间：2024-01-04

- Extraction and Monosaccharide Composition Analysis of Total Polysaccharides from
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 18, Pages: 13-16(2014)
- 作者机构：
- 作者简介：
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- DOI：10.13422/j.cnki.syfjx.2014180013
  CLC： R284
- Published：2014
- 稿件说明：
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MOU Zhen-zhen, WANG Ming-fang, GAO Wen-wen, et al. Extraction and Monosaccharide Composition Analysis of Total Polysaccharides from [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 13-16.
DOI：

MOU Zhen-zhen, WANG Ming-fang, GAO Wen-wen, et al. Extraction and Monosaccharide Composition Analysis of Total Polysaccharides from [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 13-16. DOI： 10.13422/j.cnki.syfjx.2014180013.

摘要

目的：优化桑黄子实体总多糖的提取工艺并初步分析其单糖组成。方法：采用苯酚-硫酸法测定总多糖含量。以总多糖提取率为指标，通过单因素试验和正交试验考察提取时间、提取次数、料液比对桑黄子实体总多糖微波提取工艺的影响，利用TLC初步确定不同产地和树种桑黄总多糖的单糖组成。结果：最佳提取条件为加50倍量水提取5次，每次15 min。东北桑树、东北白桦树、甘肃桑树及湖南桑树桑黄中总多糖提取率分别为5.37%，2.21%，3.04%，3.35%，均含有半乳糖和葡萄糖；山东桑树桑黄总多糖提取率2.77%，含有木糖、半乳糖、葡萄糖和乳糖；未知来源桑树桑黄中总多糖提取率3.58%，含有葡萄糖。结论：微波提取法稳定性好、提取率高。东北桑树桑黄中总多糖含量较高，可初步确定桑黄子实体总多糖中均含有葡萄糖。

Abstract

Objective: To optimize extraction process of total polysaccharides from fruiting body of Phellinus igniarius and preliminary analyze its monosaccharide composition. Method: The content of total polysaccharides was determined by phenol-sulfuric acid method.With yield of total polysaccharides as index

single factor tests and orthogonal design were adopted to optimize extraction process by taking liquid-solid ratio

extracting time and times as factors

monosaccharide composition of total polysaccharides from different origin and species of P. igniarius was determined by TLC. Result: Optimum extraction conditions were as follows:extracted 5 times with 50 times the amount of water for 15 minutes each time.Yields of total polysaccharides in P. igniarius from Dongbei mulberry

Dongbei birch

Gansu mulberry and Hunan mulberry were 5.37%

2.21%

3.04% and 3.35%

they all contained glucose and galactose;yield of total polysaccharides in P. igniarius from Shandong mulberry was 2.77%

which contained xylose

galactose

glucose and lactose;yield of total polysaccharides in P. igniarius from un-know place was 3.58%

which contained glucose. Conclusion: Microwave extraction method is stable with high extraction rate.The content of total polysaccharides in P. igniarius from Dongbei mulberry is highest by comparing with other origin and species

TLC can preliminary determine total polysaccharides from fruiting body of P. igniarius all contain glucose.

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