Optimization of Alcohol Precipitation Process for Extra-cellular Polysaccharides from Fermentative Fluid of Cordyceps

CHEN Li-hua; ZHANG Qing-hua; GUAN Yong-mei; CHENG Lei; LI Yu; ZHU Wei-feng; YANG Ming; WU De-zhi

doi:10.13422/j.cnki.syfjx.2014180020

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Optimization of Alcohol Precipitation Process for Extra-cellular Polysaccharides from Fermentative Fluid of Cordyceps

更新时间：2024-01-04

- Optimization of Alcohol Precipitation Process for Extra-cellular Polysaccharides from Fermentative Fluid of Cordyceps
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 18, Pages: 20-22(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014180020
  CLC： R284.2
- Published：2014
- 稿件说明：
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CHEN Li-hua, ZHANG Qing-hua, GUAN Yong-mei, et al. Optimization of Alcohol Precipitation Process for Extra-cellular Polysaccharides from Fermentative Fluid of Cordyceps[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 20-22.
DOI：

CHEN Li-hua, ZHANG Qing-hua, GUAN Yong-mei, et al. Optimization of Alcohol Precipitation Process for Extra-cellular Polysaccharides from Fermentative Fluid of Cordyceps[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 20-22. DOI： 10.13422/j.cnki.syfjx.2014180020.

摘要

目的：优选冬虫夏草发酵液中胞外多糖的醇沉工艺参数。方法：采用苯酚-硫酸法测定胞外多糖含量，检测波长489.4 nm。以胞外多糖质量为指标，通过正交试验考察乙醇用量、醇沉温度和时间对发酵液中胞外多糖的水提醇沉工艺的影响。结果：最佳醇沉工艺参数为加4倍量95%乙醇于20℃醇沉7.5 h；胞外多糖质量0.671 g，纯度69.32%。结论：发酵液中胞外多糖含量较高，可加以充分利用；优化的虫草胞外多糖发酵液醇沉工艺稳定可行。

Abstract

Objective: To optimize alcohol precipitation process of extra-cellular polysaccharides in fermentative fluid of Cordyceps. Method: Phenol-sulfuric acid was adopted to determine the content of extra-cellular polysaccharides with detection wavelength at 489.4 nm.Taking the content of extra-cellular polysaccharides as index

orthogonal test was adopted to optimize alcohol precipitation process with ethanol amount

alcohol precipitation temperature and time as factors. Result: Optimum alcohol precipitation process parameters were as follows:alcohol precipitation for 7.5 h with 4 times the amount of 95% ethanol at 20℃;the content of extra-cellular polysaccharides was 0.671 g with purity of 69.32%. Conclusion: The content of extra-cellular polysaccharides in fermentative fluid of Cordyceps is high and can be utilized sufficiently.This optimized alcohol precipitation is reasonable and practical for industrial production of Jinshuibao capsules.

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