Objective: Analysis and comparison the paeonol and paeoniflorinon different part of one Feng-Cortex Moutan by HPLC. Method: Using the Waters VP - ODS - C18 chromatographic column (4.6 mm×150 mm

5 μm).Mobile phase for the paeonol: methanol-water (55∶ 45)

detection wavelength 274 nm

column temperature 30℃

the flow rate of 1.0 mL·min-1. Mobile phase for thepaeoniflorin: acetonitrile - 0.1% H3PO4 (20:80)

detection wavelength 230 nm

column temperature 30℃

the flow rate of 1.0 mL·min-1. Result: Paeonol and paeoniflorin had good linear relationship in 1.073-5.365 μg and 0.593-2.965 μg

the ‘r' was 0.999 9 and 0.999 1

the average recoveriey for the paeonol was 99.96% (RSD 0.72%)

the average recovery for the paeoniflorin was 99.03% (RSD 0.49%). Conclusion: The experiments show that the content of the paeonol and paeoniflorin localed at the thinest part of the chosen Feng-Cortex Moutan is much more higher than the rest part.