Objective: The study aimed to the best harvest time through determining the content of rosmarinic acid

total diterpenoids and total flavonoids in the second cutting Linearstripe Rabdosia Herb

as well as to provide basic researching data to formulate relevant standard operating procedure (SOP) according to good agricultural practice (GAP) of Chinese medicinal materials and put them into practice. Method: HPLC and ultraviolet spectrophotometry were used to determine the content of rosmarinic acid

total diterpenoids

and total flavonoids in the second cutting Linearstripe Rabdosia Herb at different harvest time from Linearstripe Rabdosia Herb plantation base. Result: The content of rosmarinic acid varied greatly

with the difference as much as up to 10 times

while the difference of content of total diterpenoids and total flavonoids were both relatively small in the second cutting Linearstripe Rabdosia Herb at different harvest time. The contents of three active compounds were positively correlated with harvest time in Rabdosia serra

however the contents of three active compounds were fluctuated during investigation period in R. lophanthoides and R. lophanthoides (Buch.-Ham.ex D.Don) H.Hara var.graciliflora (Benth.) H.Hara. The author suggested that the best harvest time for the second cutting R. serra

R. lophanthoides

R. lophanthoides (Buch.-Ham.ex D.Don) H.Hara var. graciliflora (Benth.) H.Hara are at the 120-140th day and at the 60th day after first cutting

respectively. Conclusion: The two determination methods are simple

rapid

accurate and repeatable. All the samples used in this study were collected from Linearstripe Rabdosia Herb planting base

which are representative to evaluate the resource utilization value of the second cutting Linearstripe Rabdosia Herb. This study is of some significance to guiding the harvest of the second cutting Linearstripe Rabdosia Herb and building the Linearstripe Rabdosia Herb GAP planting base.