Simultaneous Determination of 5 Active Ingredients in Jinggu Tablets by HPLC Dual-wavelength Detection

ZENG Qi; TAN Dao-peng; CHEN Yan-ping; ZENG Wei-zhen; YAN Qi-xin; ZENG Ling-jie

doi:10.13422/j.cnki.syfjx.2014180077

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Simultaneous Determination of 5 Active Ingredients in Jinggu Tablets by HPLC Dual-wavelength Detection

更新时间：2024-01-04

- Simultaneous Determination of 5 Active Ingredients in Jinggu Tablets by HPLC Dual-wavelength Detection
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 18, Pages: 77-80(2014)
- 作者机构：
- 作者简介：
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- DOI：10.13422/j.cnki.syfjx.2014180077
  CLC： O657.72;R286.0
- Published：2014
- 稿件说明：
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ZENG Qi, TAN Dao-peng, CHEN Yan-ping, et al. Simultaneous Determination of 5 Active Ingredients in Jinggu Tablets by HPLC Dual-wavelength Detection[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 77-80.
DOI：

ZENG Qi, TAN Dao-peng, CHEN Yan-ping, et al. Simultaneous Determination of 5 Active Ingredients in Jinggu Tablets by HPLC Dual-wavelength Detection[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 77-80. DOI： 10.13422/j.cnki.syfjx.2014180077.

摘要

目的：建立HPLC 双波长法同时测定净固片中芦丁、柚皮苷、橙皮苷、新橙皮苷、槲皮素的方法。方法：依利特 Kromasil-C18色谱柱（4.6 mm×250 mm，5 μm），流动相0.2% 冰醋酸（A）-乙腈（B）梯度洗脱（0~10 min，12%~15%B；10~15 min，15%~17%B；15~25 min，17%B；25~35 min，17%~20%B；35~45 min，20%~22% B；45~60 min，22%~40%B），柱温30℃，进样量10 μL，流速1.0 mL·min-1，芦丁和槲皮素检测波长为254 nm，柚皮苷、橙皮苷和新橙皮苷检测波长为283 nm。结果：芦丁在 0.012 5~0.500 4 g·L-1（r=1.000 0），柚皮苷在0.010 0~0.400 8 g·L-1（r=0.999 9），橙皮苷在0.003 3~ 0.032 7 g·L-1（r=0.999 2），新橙皮苷在0.011 3~0.225 6 g·L-1（r=0.999 9），槲皮素在0.004 1~0.081 3 g·L-1（r=0.999 8）与峰面积具有良好的线性关系，平均回收率均在98%～102%。结论：该方法准确可靠，可行性及重复性良好，可作为净固片的质量控制方法。

Abstract

Objective: The study was conducted to establish a HPLC method for simultaneously determination of rutin

naringin

hesperidin

neohesperidin and quercetin in Jinggu tablet. Method: The separation of compounds was performed on Elite Kromasil-C18 column (4.6 mm×250 mm

5 μm)

with the mobile phase consisted of acetonitrile-0.2% acetic acid. The flow rate was set at 1.0 mL·min-1and detection wavelengths were set at 254 nm for rutin and quercetin

283 nm for naringin

hesperidin and neohesperidin. Result: The samples showed a good relationship in the ranges of 0.012 5 -0.500 4 g·L-1 for rutin

0.010 0-0.400 8 g·L-1 for naringin

0.003 3-0.032 7 g·L-1 for hesperidin

0.011 3-0.225 6 g·L-1 for neohesperidin and 0.004 1-0.081 3 g·L-1 for quercetin. All of the average recoveries were between 98% and 102%. Conclusion: The method is accurate

reliable and reproducible and can be used for the quality control of Jinggu tablet.

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