Study on Toxic Medicine Radix Podophylli and Fructus Podophylli by HPLC Fingerprint, Compare Their Toxicity

YE Yao-hui; MA Yue-xing; ZHANG En-hui; YING Ya-bin; WANG Ting

doi:10.13422/j.cnki.syfjx.2014180080

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Study on Toxic Medicine Radix Podophylli and Fructus Podophylli by HPLC Fingerprint, Compare Their Toxicity

更新时间：2024-01-04

- Study on Toxic Medicine Radix Podophylli and Fructus Podophylli by HPLC Fingerprint, Compare Their Toxicity
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 18, Pages: 80-84(2014)
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- DOI：10.13422/j.cnki.syfjx.2014180080
  CLC： O657.72;R29
- Published：2014
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YE Yao-hui, MA Yue-xing, ZHANG En-hui, et al. Study on Toxic Medicine Radix Podophylli and Fructus Podophylli by HPLC Fingerprint, Compare Their Toxicity[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 80-84.
DOI：

YE Yao-hui, MA Yue-xing, ZHANG En-hui, et al. Study on Toxic Medicine Radix Podophylli and Fructus Podophylli by HPLC Fingerprint, Compare Their Toxicity[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 80-84. DOI： 10.13422/j.cnki.syfjx.2014180080.

摘要

目的：比较桃儿七、小叶莲化学成分的异同，分析桃儿七、小叶莲的化学成分与毒性的关系，为建立鬼臼毒素类藏药的指纹图谱提供依据。方法：利用HPLC分析方法，分析桃儿七、小叶莲化学成分的差异，结合急性毒性实验方法测定桃儿七和小叶莲的LD50值，比较两药毒性差异，分析桃儿七、小叶莲鬼臼成分与毒性的关系，为桃儿七、小叶莲的质量标准提供依据。结果：标定了桃儿七与小叶莲的14个共有峰；桃儿七药材中鬼臼毒素、4'-去甲鬼臼毒素含量明显高于小叶莲，桃儿七、小叶莲LD50分别为0.088 7，0.909 8 g·mL-1。结论：桃儿七、小叶莲化学成分基本相同，但化学成分含量差异较大。桃儿七鬼臼毒素含量更高，毒性更强，在制定药材质量标准时应严格控制鬼臼毒性成分的含量。

Abstract

Objective: The aim of this study wasto compare the chemical constituents of Radix Podophylli and Fructus Podophylli

and analyze the relationship between chemical constituents and the toxicity

as well as to provide the basis for establishment of the fingerprint. Method: HPLC fingerprint was used to study the chemical composition of Radix Podophylli and Fructus Podophylli. The relationship between chemical composition and toxicity was analyzed through acute toxicity test. Result: There were 14 common chromatographic peaks for Radix Podophylli and Fructus Podophylli

the contents of Podophyllotoxin and 4'-demethylpodophyllotoxin in Radix Podophylli were higher thanin Fructus Podophylli. Median lethal dose for Radix Podophylli and Fructus Podophylli was 0.088 7

0.909 8 g·mL-1

repectively. Conclusion: Radix Podophylli and Fructus Podophyllihave the same chemical composition. But there is a great difference in content. Radix Podophylli hasstronger toxicity than Fructus Podophylli. The content of toxic components Podophyllumshould be strictly controlled in the formulation of medicine quality standard.

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