Compare of the Contents of Several Components in Flower Buds and Leaves of

XIE Jin-yan; FAN Chong-qing; SONG Xiao-mei; GU Xue-zhu; LI Rao-rao; FANG Jing

doi:10.13422/j.cnki.syfjx.2014180099

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Compare of the Contents of Several Components in Flower Buds and Leaves of

更新时间：2024-01-04

- Compare of the Contents of Several Components in Flower Buds and Leaves of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 18, Pages: 99-102(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014180099
  CLC： O657.72;R284.1
- Published：2014
- 稿件说明：
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XIE Jin-yan, FAN Chong-qing, SONG Xiao-mei, et al. Compare of the Contents of Several Components in Flower Buds and Leaves of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 99-102.
DOI：

XIE Jin-yan, FAN Chong-qing, SONG Xiao-mei, et al. Compare of the Contents of Several Components in Flower Buds and Leaves of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 99-102. DOI： 10.13422/j.cnki.syfjx.2014180099.

摘要

目的：多成分比较芫花花蕾和芫花叶的化学组成。方法：以芹菜素、木犀草素、羟基芫花素、芫花素为对照品，采用RP-HPLC方法测定芫花花蕾及芫花叶中4个成分的含量，比较2个药用部位的成分组成差异。Kromasil C18色谱柱（4.6 mm × 250 mm，5 μm），流动相甲醇-水-冰醋酸（58：42：0.5），流速0.8 mL·min-1，检测波长350 nm（木犀草素、羟基芫花素）、338 nm（芹菜素、芫花素），柱温35℃。结果：芫花花蕾中这4个成分的质量分数依次为0.017%，0.088 4%，0.069 9%，0.059 3%，芫花叶中这4个成分质量分数依次为0.011 8%，未检测到，0.012 3%，未检测到。结论：芫花叶和芫花花蕾含有两个相同成分，但含量不同；而花蕾中的两个成分在叶中未检测到，表明叶与花蕾成分不同，可根据临床治病目的分别入药。

Abstract

Objective: To compare the content of four compounds in the flower buds and leave of Daphne genkwa. Method: Apigenin

luteolin

hydroxyl genkwanin

genkwanin were used as reference. The contents of the four compounds in the flower buds and leaves were detected by RP-HPLC method. The difference between the two parts were analyzed according to the data. Kromasil C18 column (4.6 mm × 250 mm

5μm); mobile phase of methanol - water (58:42:0.5)

flow rate 0.8 mL·min-1

the detection wavelength of 350 nm (luteolin

hydroxyl genkwanin)

338 nm (apigenin

genkwanin)

the column temperature was set at 35℃. Result: The levels of these four components yuanhuacine were: 0.017%

0.088 4%

0.069 9%

0.059 3%. Daphne leaves the content of these four components as follows: 0.011 8%

not detected

0.012 3%

not detected. Conclusion: Studies showed that there were different compounds in the flower buds and leaves of the drug

so they should be used in clinic separately according to different clinical purpose.

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