Objective: To study the effect of rhynchophylline(Rhy) on glutamate receptor 1 (GluR1) subunit in hippocampus of methamphetamine-induced conditioned place preference (CPP)mice. Method: Mice were randomly divided into five groups randomly with 10 mice in each group:normal control group

methamphetamine model group

methamphetamine with Rhy (40 mg·kg-1)

methamphetamine with Rhy (80 mg·kg-1)

methamphetamine with ketamine (15 mg·kg-1). Each respective drug was administered daily for 4 days. Established place preference mice models were induced by methamphetamine

to observe the expression of GluR1 was observed by immunohistochemistry technique and Western blot. Result: Compared with normal control group

the time of mice with methamphetamine model group in non-preferred compartment increased significantly (P<0.01)

indicating that methamphetamine place preference model was successfully established. Compared with methamphetamine model group

ketamine (15 mg·kg-1)

Rhy (40 mg·kg-1)and Rhy (80 mg·kg-1) reduced the time spent on white compartment of CPP mice significantly(P<0.01).Immunohistochemistry showed that the expression of GluR1 in hippocampus was increaseding in the methamphetamine model group(P<0.01)

while in the ketamine group

low and high doseage Rhy were decreasing(P<0.01);Western blot showed that the express of GluR1 protein was significantly increased in the model group(P<0.01)

while in the ketamine group

low and high doseage Rhy group were significantly decreasing(P<0.01). Conclusion: GluR1 protein plays an important role in the formation of methamphetamine-induced place preference in mice.