Optimization of Preparation Technique of  Solid Lipid Nanoparticles by Orthogonal Test

ZHONG Xi-wen; WANG Xian-er; WU Hui-fei; WANG Ya-fei; ZHANG Wen-xia

doi:10.13422/j.cnki.syfjx.2014190015

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Optimization of Preparation Technique of Solid Lipid Nanoparticles by Orthogonal Test

更新时间：2024-01-04

- Optimization of Preparation Technique of Solid Lipid Nanoparticles by Orthogonal Test
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 19, Pages: 15-18(2014)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2014190015
  CLC： R283
- Published：2014
- 稿件说明：
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ZHONG Xi-wen, WANG Xian-er, WU Hui-fei, et al. Optimization of Preparation Technique of Solid Lipid Nanoparticles by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(19): 15-18.
DOI：

ZHONG Xi-wen, WANG Xian-er, WU Hui-fei, et al. Optimization of Preparation Technique of Solid Lipid Nanoparticles by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(19): 15-18. DOI： 10.13422/j.cnki.syfjx.2014190015.

摘要

目的：制备蛇鳞草-大黄固体脂质纳米粒并考察其形态。方法：采用HPLC测定三羽新月蕨苷A和大黄素含量，流动相乙腈（A）-0.1%甲酸水溶液（B）-水（C）梯度洗脱（0~35 min，15%~75%A，25%B，60%~0%C），检测波长226 nm。利用薄膜超声法制备蛇鳞草-大黄固体脂质纳米粒，在单因素试验基础上，以三羽新月蕨苷A和大黄素包封率为综合评价指标，采用正交试验优选处方。结果：最佳处方为加药量150 μL，硬脂酸-卵磷脂质量比1：2，吐温-80质量分数1%；纳米粒平均粒径（301±2.8）nm，Zeta电位（-19.94±3.82）mV，三羽新月蕨苷A和大黄素包封率分别为61.8%和81.2%。结论：制备的蛇鳞草-大黄固体脂质纳米粒基本呈规则球形或类球形，该工艺稳定可行。

Abstract

Objective: To prepare Prpnephrium triphyllum-Rheum officinale solid lipid nanoparticles and investigate its morphology. Method: HPLC was adopted to determine contents of emodin and triphyllin A with mobile phase of acetonitrile-0.1% formic acid-water for gradient elution and detection wavelength at 226 nm.Film-ultrasonic wave dispersion technique was used to prepare P. triphyllum-R.officinale solid lipid nanoparticles

on the basis of single factor tests

orthogonal test was employed to optimize preparation technique by taking entrapment efficiencies of emodin and triphyllin A as comprehensive evaluation index. Result: Optimal formulation was as following:extract of P. triphyllum-R.officinale 150 μL

stearic acid-soybean lecithin (1: 1)

tween-80 of 1.0%;mean particle size diameter was (301±2.8) nm

Zeta potential was (-19.94±3.82)mV

entrapment efficiencies of triphyllin A and emodin was 61.8% and 81.2%

respectively. Conclusion: These prepared P. triphyllum-R.officinale solid lipid nanoparticles show spherical

this preparation process is stable and feasible.

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