CHEN Hai-li, ZHANG Feng, XU Jing, et al. Optimization of Flash Extraction Process of Active Ingredients from Eucommiae Folium[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(19): 19-22.
DOI:
CHEN Hai-li, ZHANG Feng, XU Jing, et al. Optimization of Flash Extraction Process of Active Ingredients from Eucommiae Folium[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(19): 19-22. DOI: 10.13422/j.cnki.syfjx.2014190019.
Optimization of Flash Extraction Process of Active Ingredients from Eucommiae Folium
Objective: To optimize flash extraction process of active components from Eucommiae Folium. Method: HPLC was employed to determine contents of chlorogenic acid and geniposidic acid with mobile phase of methanol-water-glacial acetic acid(15: 85: 1)
flow rate of 1.0 mL·min-1 and detection wavelength at 237 nm.Taking yields of chlorogenic acid and geniposidic acid as index
based on single factor tests
orthogonal test was adopted to optimize extraction process with ethanol concentration
liquid-solid ratio
extracting time and times as factors. Result: Optimal process conditions were as follows:extracted thrice with 25 times the amount of 70% ethanol for 6 min each time;yields of chlorogenic acid and geniposidic acid were 1.82% and 0.47%
respectively. Conclusion: This optimized process is simple
accurate and suitable for extracting active ingredients from Eucommiae Folium.
Quality Evaluation and Suggestions on Pharmacopoeia Standard of Eucommiae Folium
Comparison of Contents of Five Effective Components in Eucommiae Folium from Different Areas
Protective Effects of Chlorogenic Acid on Liver Injury in Mice
Evaluation of Quality Analysis Method of Lonicerae Japonicae Flos Extract
Analysis on Chemical Constituents and Dynamic Accumulation of Main Components in Arctium lappa Leaves
Related Author
WANG Ping
LIANG Shaojia
ZHANG Wenwen
FENG Weihong
YI Hong
LI Chun
GUO Fengqian
SI Qin
Related Institution
Institute of Traditional Chinese Medicine(TCM),Shanghai University of TCM
Yanling DU Renkang Biotechnology Co. Ltd.
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences
Henan University of Chinese Medicine
Engineering Technology Research Center for Comprehensive Development and Utilization of Authentic Medicinal Materials in Henan Province, Henan University of Chinese Medicine