Objective: The aim of this article was to study onthe determination method of microscopic characteristic constants of pollen grains in Carthami Flos and detect the content of hydroxysafflor yellow A

and analysis of the correlation between the two sideswere analyzed. Method: Capacity analysis was used to quantify pollen grains of Carthami Flos and the content of hydroxysafflor yellow A was determined by HPLC. The data were analyzed by SPSS statistics software. Result: The microscopic characteristic constants of pollen grains and the content of hydroxysafflor yellow A in Carthami Flos were significantly correlated (positive correlation). In other words

results indicated that herbal samples with more pollen grains would contain greater amounts of the pharmacologically active components in Carthami Flos

thus be of better quality in clinical application. Conclusion: It is scientific and feasible to determine the quality of Carthami Flos is by the microscopic quantitative method. The method of based on the microscopic characteristic constants of pollen grains has been preliminarily established. It can be applied to the quality control of Carthami Flos. This approach can be further applied to other medicinal herbs

extending the use of microscopic techniques to the field of quality control of medicinal materials.