Objective: The study aimed to determine the contents of the total isoflavones

soy saponins

polysaccharide

protein

and fibrinolytic enzyme of Semen Sojae Praeparatum (SSP)from different regions. And clustering analysis was performed

to provide scientific basis for the study and evaluation of the SSP quality. Method: Genistein was used as a reference to determine the content of total isoflavones using UV spectrophotometry

with measurement wavelength at 260 nm

which showed a good linear relationship in the range of 0.996 4-14.946 mg·L-1. By using vanillin-perchloric acid colorimetric France

oleanolic acid was used as reference to determine the content of soybean saponin with measurement wavelength at 520 nm

and oleanolic acid showed a good linear relationship in the range of 2.2-11 mg·L-1. By using Phenol-sulfuric acid method glucose was used as reference to determine the content of polysaccharides with measurement wavelength at 490 nm

and glucose showed a good linear relationship in the range of 0.002 5-0.02 g·L-1. Determination of content of total protein was performed through Coomassie brilliant blue method with the detection wavelength at 595 nm. The fibrinolytic activity was determined by fibrin plate method

using urokinase as control

which showed a good linear relationship in the range of 100-10 000 U·mL-1. The cluster analysis of five active ingredients in SSP was performed by using SPSS16.0. Result: There was significant difference in the contents of main active components in commercially available SSP from different areas

with the lowest isoflavones content of 0.518 mg·g-1

and the highest of 4.511 mg·g-1; the lowest soybean saponin content was 0.873 mg·g-1

while the highest was 8.623 mg·g-1; the lowest polysaccharide content was 0.28% and the highest was 1.81%; the lowest content of total protein was 4.675%

while the highest was 36.133%. Seven SSP had no plasmin activity

however

among the other SSP with active products

the minimum of plasmin activity was 15.49 U·g-1

and the highest activity was 1 752.08 U·g-1. Cluster analysis showed good correspondence with locations. Conclusion: There are significant differences in the composition of active compounds in SSP from 21 different origins.