Simultaneous Determination Content of -asarone and -asarone in  by HPLC

LIU Meng-ting; FANG Jing; WU Hong-wei; FU Mei-hong; YANG Hong-jun

doi:10.13422/j.cnki.syfjx.2014190075

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Simultaneous Determination Content of -asarone and -asarone in by HPLC

更新时间：2024-01-04

- Simultaneous Determination Content of -asarone and -asarone in by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 19, Pages: 75-78(2014)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2014190075
  CLC： R285.5
- Published：2014
- 稿件说明：
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LIU Meng-ting, FANG Jing, WU Hong-wei, et al. Simultaneous Determination Content of -asarone and -asarone in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(19): 75-78.
DOI：

LIU Meng-ting, FANG Jing, WU Hong-wei, et al. Simultaneous Determination Content of -asarone and -asarone in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(19): 75-78. DOI： 10.13422/j.cnki.syfjx.2014190075.

摘要

目的：建立石菖蒲药材中β-细辛醚、α-细辛醚的HPLC含量测定方法，并对石菖蒲药材进行质量评价。方法：采用岛津高效液相色谱仪，对样品的前处理方法、色谱柱、检测波长、流动相等分析条件进行了优化筛选，并对分析方法的线性范围、精密度、重复性、稳定性、准确度进行考察，建立同时检测β-细辛醚、α-细辛醚的石菖蒲药材质量控制方法。结果：采用Kromasil C18色谱柱，柱温35 ℃，流动相甲醇-水（含0.1%甲酸）（70：30）等度洗脱，检测波长257 nm。回归方程β-细辛醚为Y=2×106X+205.93（r=0.999 9，0.026 96～0.539 2 μg），α-细辛醚Y=6×106X-117.6（r=0.999 9，0.016 04～0.320 8 μg）；方法精密度β-细辛醚RSD 0.119%，α-细辛醚RSD 0.116%；重复性试验β-细辛醚RSD 0.877%，α-细辛醚RSD 0.815%；稳定性试验室温下20 h内样品中β-细辛醚RSD 0.42%，α-细辛醚RSD 0.25%；准确度β-细辛醚平均回收率为97.7%（RSD 1.9%），α-细辛醚平均回收率为102.3%（RSD 1.5%）。所收集的石菖蒲样品中β-细辛醚含量1.12%~3.51%，α-细辛醚含量0.03%~1.01%。结论：该方法快速、准确、重复性好，可用于石菖蒲药材的质量评价。

Abstract

Objective: A method of high-performance liquid chromatography was developed to evaluate the quality of a traditional Chinese medicine Acorus tatarinowii through analyzing the content of β-asarone and α-asarone. Method: The conditions of sample preparation

chromatography column

detected wavelength and mobile phase were optimized. The linear ranges

precision

reproducibility

stability and accuracy of the method were evaluated based on a Shimadzu HPLC system. And six batches of A. tatarinowii were detected. Result: The determination was performed on a Kromasil C18 column at 257 nm wavelength.The mobile phase was isocratic elution of methanol-water(0.1% formic acid)(70:30). The flow rate was set at 1.0 mL·min-1

and column temperature was at 35 ℃.The regression equation of β-asarone was Y= 2×106X+205.93(r=0.999 9

0.026 96-539 2 μg)

and that of α-asarone was Y=6×106X-117.6 (r=0.999 9

0.016 04-0.320 8 μg); the RSDs of precision were 0.119% (β-asarone) and 0.116% (α-asarone); the RSDs of repeatability were 0.877% (β-asarone) and 0.815% (α-asarone); the RSDs of stability test within 20 hours at room temperature were 0.42% (β-asarone) and 0.25% (α-asarone); the average recovery of β-asarone was 97.7% (RSD 1.9% )

for α-asarone it was 102.3% (RSD 1.5%). The content of β-asarone and α-asarone in A. tatarinowii samples were in the range of 1.12%-3.51% and 0.03%-1.01%

respectively. Conclusion: This method is convenient

accurate and reliable

which can be applied to be a quality evaluation method for A. tatarinowii.

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