Determination of Cirsimarin in Leaves of by HPLC

LI Chun-yang; ZHANG Ping; YUAN Xu-jiang

doi:10.13422/j.cnki.syfjx.2014190079

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Determination of Cirsimarin in Leaves of by HPLC

更新时间：2024-01-04

- Determination of Cirsimarin in Leaves of by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 19, Pages: 79-81(2014)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2014190079
  CLC： R284.1
- Published：2014
- 稿件说明：
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LI Chun-yang, ZHANG Ping, YUAN Xu-jiang. Determination of Cirsimarin in Leaves of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(19): 79-81.
DOI：

LI Chun-yang, ZHANG Ping, YUAN Xu-jiang. Determination of Cirsimarin in Leaves of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(19): 79-81. DOI： 10.13422/j.cnki.syfjx.2014190079.

摘要

目的：建立相思子叶中滨蓟黄苷含量的HPLC测定方法。方法：采用高效液相色谱法，Hypersil BDS C18色谱柱（4.6 mm×150 mm，5 μm），流动相甲醇（A）-0.2%甲酸水溶液（B），梯度洗脱（0~25 min，15%~70%A），检测波长330 nm，流速1.0 mL·min-1。结果：滨蓟黄苷在0.327~3.27 μg与其峰面积呈良好的线性关系，线性回归方程为Y=1 933.4 X +57.897（r=0.999 5），平均加样回收率为99.46%（RSD 1.78%）。结论：该方法简便准确、分离度好、专属性强，可用于相思子叶中滨蓟黄苷的含量测定。

Abstract

Objective: The aim of this study was tTo establish an HPLC method for the determination of cirsimarin in the leaves of Abrus precatorius. Method: HPLC was performed on Hypersil BDS C18 column (4.6 mm×150 mm

5 μm )

and the mobile phase was methanol(A)-0.2% formic acid solution(B) with the gradient elution program(0-25 min

15%-70% A). The detection wavelength was set at 330 nm

the flow rate was 1.0 mL·min-1. Result: Cirsimarin showed a good linear relationship from 0.327 to 3.27 μg(r=0.999 5)

and the regression equation was Y=1933.4X+ 57.897 (r=0.999 5). The average recovery was 99.46% with RSD of 1.78%. Conclusion: The HPLC method is simpley and accurate with agood specificity. It can be used to determine the content of cirsimarin in the leaves of A. precatorius.

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