Determination of Content of Quercetin and Total Flavonoids in Trichosanthis Pericarpium from Different Areas

WANG Zhu-zhi; WANG Xiao-hua; ZHU Yu; WANG Chao-qun

doi:10.13422/j.cnki.syfjx.2014190086

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Determination of Content of Quercetin and Total Flavonoids in Trichosanthis Pericarpium from Different Areas

更新时间：2024-01-04

- Determination of Content of Quercetin and Total Flavonoids in Trichosanthis Pericarpium from Different Areas
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 19, Pages: 86-89(2014)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2014190086
  CLC： R284.1
- Published：2014
- 稿件说明：
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WANG Zhu-zhi, WANG Xiao-hua, ZHU Yu, et al. Determination of Content of Quercetin and Total Flavonoids in Trichosanthis Pericarpium from Different Areas[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(19): 86-89.
DOI：

WANG Zhu-zhi, WANG Xiao-hua, ZHU Yu, et al. Determination of Content of Quercetin and Total Flavonoids in Trichosanthis Pericarpium from Different Areas[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(19): 86-89. DOI： 10.13422/j.cnki.syfjx.2014190086.

摘要

目的：建立瓜蒌皮药材中槲皮素和总黄酮的含量测定方法，并测定不同产地瓜蒌皮药材中槲皮素及总黄酮的含量。方法：分别用不同浓度的乙醇和甲醇做为槲皮素的提取溶剂，采用浸渍提取、超声提取、回流提取3种不同提取方法提取槲皮素，采用HPLC测定槲皮素含量；用75%乙醇提取瓜蒌皮药材中总黄酮，采用紫外分光光度法测定总黄酮含量。结果：槲皮素用甲醇回流提取率较高，江苏所产瓜蒌皮药材中槲皮素含量较高，安徽所产瓜蒌皮药材中总黄酮含量较高。结论：此含量测定方法重复性好，准确可靠。不同产地瓜蒌皮药材中槲皮素及总黄酮含量差别较大。

Abstract

Objective: The purpose of the study was to establish a method for the determination of the content of quercetin and total flavonoids in Trichosanthis Pericarpium

and to determine the content of quercetin and total flavonoids in Trichosanthis Pericarpium from different areas. Method: Diverse concentrations of ethanol and methanol were chosen as the extraction solvents for quercetin while it was extracted by immersion extraction

ultrasonic extraction

and refluxing process

then the content was determined by HPLC. The content of total flavonoids was determined using ultraviolet spectrophotometry after it was extracted by ethanol (75%). Result: Refluxing process with methanol proved to be the optimal extraction method for quercetin. The Pericarpium trichosanthis from Jiangsu province showed higher content of quercetin

additionally

the content of total flavonoids in Trichosanthis Pericarpium from Anhui province was much more than other areas. Conclusion: The method established to determine the content of quercetin and total flavonoids in Trichosanthis Pericarpium in this study exhibits good reproducibility and accuracy. The contents of quercetin and total flavonoids in Trichosanthis Pericarpium were significantly different in different areas.

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