Standard of Quality Control of Mailuotong Jiaonang

TAN Qin; SHEN Ting; JIN Fang; GUO Qing; YAO Zhong-qing

doi:10.13422/j.cnki.syfjx.2014190093

您当前的位置：

首页 >

文章列表页 >

Standard of Quality Control of Mailuotong Jiaonang

更新时间：2024-01-04

- Standard of Quality Control of Mailuotong Jiaonang
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 19, Pages: 93-96(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014190093
  CLC： R286.0
- Published：2014
- 稿件说明：
移动端阅览
TAN Qin, SHEN Ting, JIN Fang, et al. Standard of Quality Control of Mailuotong Jiaonang[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(19): 93-96.
DOI：

TAN Qin, SHEN Ting, JIN Fang, et al. Standard of Quality Control of Mailuotong Jiaonang[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(19): 93-96. DOI： 10.13422/j.cnki.syfjx.2014190093.

摘要

目的：提高脉络通胶囊的质量标准。方法：采用TLC法鉴别制剂中丹参、山楂、川芎、地龙，采用HPLC同时测定葛根素、芦丁、丹酚酸B含量。色谱条件为：Kromasil 100-5C18色谱柱（4.6 mm×250 mm，5 μm），柱温35 ℃，以甲醇（A）-1%冰醋酸溶液梯度洗脱（0～45 min，25%~45% A，45～55 min，45%~25%A），流速1.0 mL·min-1，检测波长254 nm。结果：TLC可鉴别丹参、山楂、川芎、地龙。葛根素的进样量在0.130 6～1.959 6 μg呈现良好的线性，r=0.999 9；平均加样回收率97.77%，RSD 0.70%；芦丁的进样量在1.054 2～15.813 6 μg呈现良好的线性，r=0.999 9；平均加样回收率98.66%，RSD 0.89%；丹酚酸B的进样量在0.184 9～2.773 2 μg呈现良好的线性，r=0.999 5；平均加样回收率99.55%，RSD 1.15%。结论：该法专属性强，重复性好，结果准确，可用于控制脉络通胶囊质量。

Abstract

Objective: To improve the standard of quality control of Mailuotong Jiaonang. Method: TLC identify Salviae miltiorrhizae radix et rhizoma

Crataegi fructus

Chuanxiong rhizome

Pheretima.The Kromasil 100-5C18(4.6 mm×250 mm

5 μm) was column adopted with the temperature at 35 ℃

mobile phase A was methanol

mobile phase B was 1% glacial acetic acid solution(0-45 min

25%-45% A; 45-55 min

45%-25%A)the flow rate at 1.0 mL·min-1

the detection wavelength was 254 nm. Result: The calibration curve of puerarin was linear in the range of 0.130 6-1.959 6 μg with the correlation of 0.999 9(n=9). The average recovery of puerarin was 97.77%

RSD 0.70%(n=6). The calibration curve of rutin was linear in the range of 1.054 2-15.813 6 μg with the correlation of 0.999 9(n=9). The average recovery of rutin was 98.66%

RSD 0.89%(n=6). The calibration curve of salvia acid B was linear in the range of 0.184 9-2.773 2 μg with the correlation of 0.999 5(n=9). The average recovery of salvia acid B was 99.55%

RSD 1.15%(n=6). Conclusion: The method is specific

reliable and accurate

so it can be used for the quality control of Mailuotong Jiaonang.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Study on the Quality Control Standard of Huoxue Yangyin Granules

Studies on Quality Standard for Danqi Quyu Zhitong Granules

Effect of Salvianolic Acid B Combined with Puerarin on Pyroptosis of SH-SY5Y Cells Injured by Oxygen-glucose Deprivation/Reoxygenation

Study on Quality Standard of Zhifukang Capsule

Related Author

CHEN Shi-cui

JU Jian-ming

WANG Zhao-yu

BAI Xiu-yun

BAI Hai-yu

WANG Wei-ming

WANG Zhao-yu

BAI Xiu-yun

Related Institution

Heilongjiang Academy of Traditional Chinese Medicine

Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences and Peking Union Medical College

Hubei Institute for Food and Drug Control

Shanxi Renyuantang Pharmaceutical Co.,Ltd.

Shanxi University of Traditional Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰