ZHAO Hong, CHAI Gui-fang, WANG Qiu-hong, et al. Isolation,Purification and Monosaccharide Analysis of a Polysaccharide from Plantaginis Semen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(19): 97-100.
DOI:
ZHAO Hong, CHAI Gui-fang, WANG Qiu-hong, et al. Isolation,Purification and Monosaccharide Analysis of a Polysaccharide from Plantaginis Semen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(19): 97-100. DOI: 10.13422/j.cnki.syfjx.2014190097.
Isolation,Purification and Monosaccharide Analysis of a Polysaccharide from Plantaginis Semen
Objective: The aim of this study was to isolate and purify the polysaccharide from Plantaginis Semen and to analyse the monosaccharide composition of the polysaccharide. Method: The crude polysaccharide from hot water extraction was subject to remove protein and preliminary isolated through Amberlite FPA90-Cl-+Amberlite IRC-84
purified by DEAE-cellulose chromatography and Sephacryl S-400 chromatography
a polysaccharide fraction named Plantago depressa L. polysaccharide B10(PP-B10) was obtained. The structural characterization and purity of PP-B10 were identified by Ultraviolet-visible Spectrophotometry and fourier transform infrared spectroscopy (FTIR). The monosaccharide composition analyses of PP-B10 was conducted by PMP pre-column derivatization high performance liquid chromatography. Result: A acidic polysaccharide fraction in high purity named PP-B10 was obtained from Plantaginis Semen and it was mainly composed of mannose
ribose
galacturonic acid
galactose
xylose
arabinose and fucose. Conclusion: PP-B10 was a novel polysaccharide with new monosaccharide composition which was found in Plantaginis Semen.
Effect of Polysaccharides from Plantaginis Semen on Renal Injury and Gut Microbiota in Rats with Membranous Nephropathy
Effect of Step Alcohol Precipitation on Compositions and Antioxidant Activity of Maca Polysaccharide
Polygonati Odorati Rhizoma Polysaccharides Ameliorate Hyperlipidemia in Mice by Regulating Gut Microbiota
Oral Absorption and Labeling Techniques of Traditional Chinese Medicine Polysaccharides: A Review
Structural Characteristics and Antioxidant Activity Analysis of Polysaccharides from Pinelliae Rhizoma and Its Processed Products Before and After Hydrolysis (Enzymolysis) by Sugar Spectrum
Related Author
Hong ZHAO
Chen CHEN
Yan ZHAO
Wei-wei TANG
Qi GAO
Ling-zhou KONG
Deng-jun YU
Yu ZHANG
Related Institution
College of Pharmacy,Jiamusi University
Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province
School of Pharmacy, Hunan University of Chinese Medicine