Objective: To observe protective effect of Modified Sini decoction on immunological liver injury in mice induced by concanavalin A(Con A)and the role of T lymphocyte subsets in which. Method: The mouse were randomly divided into normal saline(NS)group

Con A group

bifendate group(LB

150 mg·kg-1)

Modified Sini decoction low

high dosage group(120

240 mg·kg-1).All groups

except for the NS group

were given Con A aqueous solution(18 mg·kg-1)via tail vein injection 1 hour after drug administration at d1 and d3

peripheral blood was collected 12 h after Con A was injected. The percentage of T lymphocyte subsets CD4+

CD8+cells were detected in plasma by a flow cytometer

using enzyme-linked imunosorbent assay(ELISA) for determination the serum cytokines:interferon-γ(IFN-γ)

tumor necrosis factor alpha(TNF-α)

interleukin(IL)-10 and activities of alanine aminotransferase(ALT)

aspartate aminotransferase(AST). Result: The activity of AST and ALT of Modified Sini decoction treatment groups significantly dropped in mice of Con A-mediated liver injury;SND-treated groups could significantly reduced the liver injury model group had elevated inflammatory cytokines IFN-γ or TNF-α level(P<0.05

P<0.01);Modified Sini decoction groups could improve the liver injury model group had lowed T-cell subsets CD4+

CD8+rate(P<0.05). Conclusion: Modified Sini decoction is effective in protecting immunological liver injury in mice induced by Con A and this mechanism maybe closely associated with its action in regulating the T-cell subsets and in reducing the inflammatory cytokines.