Extraction Process of -Cyclodextrin in Microcapsule Aqueous Two-phase System for Volatile Oil from Chuanxiong Rhizoma and Asari Radix et Rhizoma by Orthogonal Test

MU Qi-yun; LAN Tao-hua; XU Fang-fang; RUAN Xin-min

doi:10.13422/j.cnki.syfjx.2014200001

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Extraction Process of -Cyclodextrin in Microcapsule Aqueous Two-phase System for Volatile Oil from Chuanxiong Rhizoma and Asari Radix et Rhizoma by Orthogonal Test

更新时间：2024-01-04

- Extraction Process of -Cyclodextrin in Microcapsule Aqueous Two-phase System for Volatile Oil from Chuanxiong Rhizoma and Asari Radix et Rhizoma by Orthogonal Test
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 20, Pages: 1-5(2014)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2014200001
  CLC： R282.4;R283.6;R284.1
- Published：2014
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MU Qi-yun, LAN Tao-hua, XU Fang-fang, et al. Extraction Process of -Cyclodextrin in Microcapsule Aqueous Two-phase System for Volatile Oil from Chuanxiong Rhizoma and Asari Radix et Rhizoma by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 1-5.
DOI：

MU Qi-yun, LAN Tao-hua, XU Fang-fang, et al. Extraction Process of -Cyclodextrin in Microcapsule Aqueous Two-phase System for Volatile Oil from Chuanxiong Rhizoma and Asari Radix et Rhizoma by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 1-5. DOI： 10.13422/j.cnki.syfjx.2014200001.

摘要

目的：优选川芎和细辛挥发油的β-环糊精（β-CD）微囊萃取工艺条件。方法：选择β-CD和硫酸钠作为双水相萃取剂，以挥发油收率、甲基丁香酚和苯酞类物质（以藁本内酯计）质量的综合评分为指标，通过正交试验考察萃取液中β-环糊精和硫酸钠的质量浓度、提取方式、萃取温度对微囊萃取工艺的影响。采用GC-MS分析挥发油的组分，以邻二甲苯和邻苯二甲酸二丁酯为内标物。结果：最佳萃取工艺条件为加3倍量40%乙醇室温下浸泡24 h，60 ℃热浸60 min，抽滤，提取液中加入β-CD和硫酸钠至质量浓度分别为300，50 g ·L-1，50 ℃振荡萃取30 min；挥发油收率86.54%，挥发油中甲基丁香酚和苯酞类物质（以藁本内酯计）质量分别为75.88，353.91 mg。结论：β-CD微囊双水相萃取及包合川芎和细辛挥发油的工艺合理可行，适合于采用β-CD包合的挥发油制剂工业化生产。

Abstract

Objective: To optimize β-cyclodextrin(β-CD) microcapsule extraction process of volatile oil from Chuanxiong Rhizoma and Asari Radix et Rhizoma. Method: β-CD and sodium sulfate were employed as extraction agents in aqueous two-phase system

with composite score of volatile oil yield

quality of methyl eugenol and phthalides (in the content of ligustilide) as index

L9 (34) orthogonal test was adopted to optimize extraction and inclusion technology of volatile oil from Chuanxiong Rhizoma and Asari Radix et Rhizoma by β-CD microcapsule

the concentration of β-CD and sodium sulfate in extract

extraction methods and temperature were selected as main influence factors.Taking O-xylene and dibutyl phthalate as internal standards

components of volatile oil was analyzed by GC-MS. Result: Optimal extraction process conditions were as follows:soaked 24 h with 3 times the amount of 40% ethanol

heated to temperature of 60 ℃ and stirred for 60 min

then vacuum suction filtrated

β-CD and sodium sulfate were added to filtrate to the concentration of 300 g·L-1 and of 50 g·L-1

respectively;stirred and extracted for 30 min at 50 ℃.Under these conditions

yield of volatile oil was 86.54%

contents of methyl eugenol and phthalides (in the content of ligustilide) were 75.88 mg and 353.91 mg. Conclusion: Technologies for extraction and inclusion of volatile oil from Chuanxiong Rhizoma and Asari Radix et Rhizoma are feasible and suitable for industrial production of volatile oil preparations for β-CD inclusion.

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