LUO Jing, HE Zhong-yan, PEI Jin, et al. Purification Technology of Limonoids in Citri Reticulatae Semen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 13-16.
DOI:
LUO Jing, HE Zhong-yan, PEI Jin, et al. Purification Technology of Limonoids in Citri Reticulatae Semen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 13-16. DOI: 10.13422/j.cnki.syfjx.2014200013.
Purification Technology of Limonoids in Citri Reticulatae Semen
Objective: To optimize purification process of limonoids in Citri Reticulatae Semen by macroporous resin. Method: Static adsorption and desorption tests were adopted to select optimal type from 16 kinds of macroporous resin with different manufacturers and polarities.Purification process was optimized by single factor tests. Result: HPD-722 macroporous resin was selected
optimal purification process was as following:the concentration of sample solution between 3.503 3-5.403 4 g·L-1
sampling speed of 1-2 BV·h-1
pH of sample solution 6
washed impurity by 1 BV of water and eluted by 6 BV of 80% ethanol with elution rate of 2 BV·h-1
eluent pH of 7;under these conditions
purity of limonoids was 82.82%
including limonin of 22.53% and nomilin of 45.90%. Conclusion: Limonoids can be purified by HPD-722 macroporous resin
this process can effectively improve the purity of limonoids.
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