Determination of Equilibrium Solubilities of Total Saponins Extract from  in Different Mediums

LIU Yan; HE Bing; XIONG Wei; LI Chun-hong; TIAN Ji

doi:10.13422/j.cnki.syfjx.2014200051

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Determination of Equilibrium Solubilities of Total Saponins Extract from in Different Mediums

更新时间：2024-01-04

- Determination of Equilibrium Solubilities of Total Saponins Extract from in Different Mediums
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 20, Pages: 51-53(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014200051
  CLC： R282.4;R283.6;R284.1
- Published：2014
- 稿件说明：
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LIU Yan, HE Bing, XIONG Wei, et al. Determination of Equilibrium Solubilities of Total Saponins Extract from in Different Mediums[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 51-53.
DOI：

LIU Yan, HE Bing, XIONG Wei, et al. Determination of Equilibrium Solubilities of Total Saponins Extract from in Different Mediums[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 51-53. DOI： 10.13422/j.cnki.syfjx.2014200051.

摘要

目的：测定楤木总皂苷在水，0.1 mol ·L-1盐酸和不同pH磷酸盐缓冲液中的平衡溶解度，为楤木总皂苷提取物的剂型设计提供实验依据。方法：以楤木皂苷A为指标，采用HPLC测定楤木皂苷A在不同介质中平衡溶解度，流动相乙腈（A）-0.1%磷酸溶液（B）梯度洗脱（0~18 min，35%~43%A；18~19 min，43%A；19~25 min，43%~35%A），检测波长205 nm。结果：37 ℃时，楤木皂苷A在水，0.1 mol ·L-1盐酸和pH 2，4，6，7，8，10的磷酸盐缓冲液中平衡溶解度分别为1.780，0.241，0.420，0.472，1.196，1.746，0.180，0.242 g ·L-1。结论：楤木总皂苷提取物微溶于水，但受介质的pH影响大。

Abstract

Objective: To establish a method for determination equilibrium solubilities of total saponins extract from Aralia chinensis in different mediums. Method: Taking araloside A as index

an HPLC method was performed and adopted to detect the concentration of araloside A in water

0.1 mol·L-1 HCl and different phosphate buffer with pH of 2

10 at 37 ℃

mobile phase of acetonitrile(A)-0.1% phosphoric acid solution(B) for gradient elution (0-18 min

35%-43%A;18-19 min

43%A;19-25 min

43%-35%A)

detection wavelength was set at 205 nm. Result: Equilibrium solubilities of araloside A in different mediums were 1.780

0.241

0.420

0.472

1.196

1.746

0.180

0.242 g·L-1. Conclusion: Total saponins extract from A. chinensis dissolves in water

its equilibrium solubility is influenced greatly by pH values.

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