Determination of Two Anthraquinones in Hedyotidis Herba by HPLC-DAD

CAO Guang-shang; YANG Pei-min; ZHANG Jia-yu; LI Fang; NI Jian; GAO Peng

doi:10.13422/j.cnki.syfjx.2014200054

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Determination of Two Anthraquinones in Hedyotidis Herba by HPLC-DAD

更新时间：2024-01-04

- Determination of Two Anthraquinones in Hedyotidis Herba by HPLC-DAD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 20, Pages: 54-56(2014)
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- DOI：10.13422/j.cnki.syfjx.2014200054
  CLC： R284.1
- Published：2014
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CAO Guang-shang, YANG Pei-min, ZHANG Jia-yu, et al. Determination of Two Anthraquinones in Hedyotidis Herba by HPLC-DAD[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 54-56.
DOI：

CAO Guang-shang, YANG Pei-min, ZHANG Jia-yu, et al. Determination of Two Anthraquinones in Hedyotidis Herba by HPLC-DAD[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 54-56. DOI： 10.13422/j.cnki.syfjx.2014200054.

摘要

目的：建立HPLC-DAD测定白花蛇舌草药材中2个蒽醌类成分2-甲基-3-甲氧基蒽醌（蒽醌Ⅰ）、2，3-二甲氧基-6-甲基蒽醌（蒽醌Ⅱ）含量的方法。方法：采用ZORBAX Extend-C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-0.5%磷酸溶液（48：52），检测波长265 nm，流速1.0 mL ·min-1，柱温25 ℃。结果：蒽醌Ⅰ在0.998~9.98 μg（r=0.999 6）、蒽醌Ⅱ在0.675~6.75 μg（r=0.999 9）线性关系良好，平均加样回收率分别为 99.87%（RSD 1.13%）和99.51%（RSD 0.69%）。不同批次的白花蛇舌草药材中蒽醌Ⅰ和蒽醌Ⅱ成分含量差异较大，蒽醌Ⅰ和蒽醌Ⅱ的比例也无明显规律。结论：该方法简单、准确，适用于白花蛇舌草药材中2-甲基-3-甲氧基蒽醌和2，3-二甲氧基-6-甲基蒽醌的质量控制。

Abstract

Objective: The aim of this study was to establish an HPLC-DAD method for the determination of 2-methy-3-methoxyanthraquinone

3-dimethoxy-6-methyanthraquinone in Hedyotidis Herba. Method: The assay was performed on a ZORBAX Extend-C18 column(4.6 mm×250 mm

5 μm) eluted with acetonitrile -0.5% phosphate(48:52).The detection wavelength was 265 nm

the flow rate was 1.0 mL·min-1

and the column temperature was set at 25 ℃. Result: The calibration curves were linear in the ranges of 0.998-9.98 μg(r=0.999 6)for anthraquinone I and 0.675-6.75 μg(r=0.999 9)for anthraquinone II. The average recoveries of anthraquinone I and anthraquinone II were 99.87%(RSD 1.13%)and 99.51%(RSD 0.69%)

respectively. Conclusion: The method is simple and accurate for the quality control of 2-methy-3-methoxyanthraquinone and 2

3-dimethoxy-6-methyanthraquinone in Hedyotidis Herba.

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