Simultaneous Determination of Eight Bioactive Components in Lonicerae Japonicae Flos by Quantitative Analysis of Multi-components by Single Marker

WANG Ling-na; LIU Hong-yan; ZHANG Jin; LI Jia; ZHANG Yong-qing

doi:10.13422/j.cnki.syfjx.2014200057

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Simultaneous Determination of Eight Bioactive Components in Lonicerae Japonicae Flos by Quantitative Analysis of Multi-components by Single Marker

更新时间：2024-01-04

- Simultaneous Determination of Eight Bioactive Components in Lonicerae Japonicae Flos by Quantitative Analysis of Multi-components by Single Marker
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 20, Pages: 57-61(2014)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2014200057
  CLC： R284.1
- Published：2014
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WANG Ling-na, LIU Hong-yan, ZHANG Jin, et al. Simultaneous Determination of Eight Bioactive Components in Lonicerae Japonicae Flos by Quantitative Analysis of Multi-components by Single Marker[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 57-61.
DOI：

WANG Ling-na, LIU Hong-yan, ZHANG Jin, et al. Simultaneous Determination of Eight Bioactive Components in Lonicerae Japonicae Flos by Quantitative Analysis of Multi-components by Single Marker[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 57-61. DOI： 10.13422/j.cnki.syfjx.2014200057.

摘要

目的：采用“一测多评法”（QAMS）与外标法（ESM）同时测定金银花药材中8种活性成分（新绿原酸、绿原酸、隐绿原酸、芦丁、木犀草苷、异绿原酸B、异绿原酸A、异绿原酸C）的含量，并进行比较，确定“一测多评法”在金银花药材质量控制中的应用价值。方法：以绿原酸为参照物，采用ZORBAX SB-C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-0.1%磷酸梯度洗脱，流速1 mL ·min-1，柱温30 ℃，检测波长325，350 nm，以特征图谱鉴别8种活性成分。结果：两种方法测得的活性成分含量间无显著性差异，RSD<5%。结论：“一测多评法”测定金银花8种活性成分含量结果可信，可用于金银花药材质量评价。

Abstract

Objective: The aim of this study was to establish a method for simultaneous assay of eight kinds of active ingredients in Lonicerae Japonicae Flos (neochlorogenic acid

chlorogenic acid

cryptochlorogenic acid

rutin

cynaroside

isochlorogenic acid B

isochlorogenic acid A

isochlorogenic acid C) by quantitative analysis of multi-components by single marker (QAMS) and external standard method (ESM)

and determine the application value of QAMS in the quality control of Lonicerae Japonicae Flos. Method: The chromatographic separation was carried out on a ZORBAX SB-C18 (4.6 mm×250 mm

5 μm) column with gradient elution of acetonitrile and 0.1% phosphoric acid at a flow rate of 1 1 mL·min-1. The detection wave-length was 325 nm and 350 nm.A characteristic spectrum was used for identification of eight components. By using chlorogenic acid as reference

the relative corrective factors (RCF) of eight components were calculated.The method was evaluated by comparison of the quantitative results through external standard method and QAMS method. Result: The results from QAMS method were not significantly different from those from external standard method. Conclusion: The method is of high sensitivity and nice reproducibility

and it could be used to control the quality of Lonicerae Japonicae Flos with QAMS.

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