Determination of Paeoniflorin, Ferulic Acid and Hesperidin in Ruanjian Xiaoying Granules by HPLC

FAN Hui; TIAN Yuan

doi:10.13422/j.cnki.syfjx.2014200077

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Determination of Paeoniflorin, Ferulic Acid and Hesperidin in Ruanjian Xiaoying Granules by HPLC

更新时间：2024-01-04

- Determination of Paeoniflorin, Ferulic Acid and Hesperidin in Ruanjian Xiaoying Granules by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 20, Pages: 77-79(2014)
- 作者机构：
- 作者简介：
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- DOI：10.13422/j.cnki.syfjx.2014200077
  CLC： O657.72;R284
- Published：2014
- 稿件说明：
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FAN Hui, TIAN Yuan. Determination of Paeoniflorin, Ferulic Acid and Hesperidin in Ruanjian Xiaoying Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 77-79.
DOI：

FAN Hui, TIAN Yuan. Determination of Paeoniflorin, Ferulic Acid and Hesperidin in Ruanjian Xiaoying Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 77-79. DOI： 10.13422/j.cnki.syfjx.2014200077.

摘要

目的：建立软坚消瘿颗粒中芍药苷、阿魏酸、橙皮苷的含量测定方法，为其质量标准的建立提供依据。方法：采用高效液相色谱波长切换法同时测定芍药苷、阿魏酸、橙皮苷含量，Agilent TC-C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-0.2%冰醋酸（18：82），流速1.0 mL ·min-1，柱温25 ℃，检测波长0~12 min为230 nm，12~20 min为325 nm，20~40 min为283 nm。结果：3 种活性成分实现良好分离，芍药苷在0.061 7~1.541 7 mg线性关系良好，r=0.999 9，平均回收率为97.25%，RSD 1.45%；阿魏酸在0.005 9~0.146 7 mg线性关系良好，r=0.999 7，平均回收率为98.66%，RSD 1.45%；橙皮苷在0.136 7 ~3.416 7 mg线性关系良好，r=0.999 6，平均回收率为97.55%，RSD 1.92%。结论：该方法简便、准确、可靠，重复性好，为控制软坚消瘿颗粒的质量提供依据。

Abstract

Objective: The aim of this stydy was to establish a method to determine paeoniflorin

ferulic acid and hesperidin in Ruanjian Xiaoying granules. Method: The chromatographic separation was performed on Agilent TC-C18(4.6 mm×250 mm

5 μm) column. The mobile phase was acetonitrile-0.2% acetic acid(18:82). The flow rate was 1.0 mL·min-1and column temperature was maintained at 25 ℃. The detection wavelength was set at 230 nm within 0-12 min

325 nm within 12-20 min

283 nm within 20-40 min. Result: The three compounds were separated well. The linear ranges of paeoniflorin

ferulic acid and hesperidin were 0.061 7-1.541 7 mg(r=0.999 9

average recovery was 97.25%

RSD 1.45%)

0.005 9-0.146 7 mg(r=0.999 7

average recovery was 98.66%

RSD 1.45%) and 0.136 7-3.416 7 mg(r=0.999 6

average recovery was 97.55%

RSD 1.92%). Conclusion: The method is rapid

simple and accurate

and can be used for quality control of Ruanjian Xiaoying granules.

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