Transport Behavior of Aconitine in Caco-2 Cell Monolayer Model

ZHENG Qin; ZHOU Huan; XIONG Wen-hai; HU Shuang; XUE Xin; HU Peng-yi; YANG Ming

doi:10.13422/j.cnki.syfjx.2014200121

您当前的位置：

首页 >

文章列表页 >

Transport Behavior of Aconitine in Caco-2 Cell Monolayer Model

更新时间：2024-01-04

- Transport Behavior of Aconitine in Caco-2 Cell Monolayer Model
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 20, Pages: 121-124(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014200121
  CLC： R285.5
- Published：2014
- 稿件说明：
移动端阅览
ZHENG Qin, ZHOU Huan, XIONG Wen-hai, et al. Transport Behavior of Aconitine in Caco-2 Cell Monolayer Model[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 121-124.
DOI：

ZHENG Qin, ZHOU Huan, XIONG Wen-hai, et al. Transport Behavior of Aconitine in Caco-2 Cell Monolayer Model[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 121-124. DOI： 10.13422/j.cnki.syfjx.2014200121.

摘要

目的：研究乌头碱在Caco-2细胞模型上的转运行为。方法：采用MTT法确定乌头碱在Caco-2细胞转运过程中的安全浓度范围，以乌头碱累积转运量及表观渗透系数Papp为指标，采用高效液相色谱法对成分进行检测，考察不同浓度、不同孵育时间乌头碱在Caco-2细胞模型上的双向转运。结果：乌头碱累积转运量与孵育时间、给药浓度呈正相关，不同浓度乌头碱Papp保持恒定，无统计学差异，且外排作用明显强于吸收，外排比接近1.5，Papp约为1×10-5cm ·s-1。结论：乌头碱以被动转运为主，且可能存在外排蛋白的参与，为吸收良好的一类药物。

Abstract

Objective: To study the transport behavior of aconitine in the Caco-2 cell monolayer model. Method: MTT method was employed to confirm the safe concentration of aconitine towards Caco-2 cell.Aconitine concentrations in the samples were analyzed by high performance liquid chromatography (HPLC).The apparent permeability(Papp)value and the total amount of transport were calculated. Bi-direction transport behavior of aconitine by incubating in different concentration and time were studied. Result: The transport amount of aconitine was positively correlated with the incubation time and drug concentration on the Caco-2 monolayer model. The Papp values in both apical to basal (AP-BL)and basal to apical (BL-AP) directions were stable with no significant differences in selected concentrations. The BL-AP flux of aconitine was more than 1.5-fold higher than AP-BL flux(Papp 1×10-5cm·s-1). Conclusion: The results suggested that the aconitineis well absorbed through the Caco-2 monolayer model by a passive transportation. Besides

efflux protein may be involved in the transport process.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Recovery and Utilization of Alkaloids in Processing Wastewater of Aconiti Radix

To Analyse the Toxicity of Tongmai-Sini-Tang

Determination of Aconitine in Tissues of Rats by HPLC

The Determination of Ester-alkaloids and Mercury Sulfide in Ershiwuweishanhu Pills

Progress in Research on Absorption of Breviscapine and Strategies for Absorption-Enhancement

Related Author

Jian-ping YI

Jing DU

He MENG

Yu-ming CHI

Jia-fu CHEN

GE Er-ning

LI Wen-dong

MA Chen

Related Institution

Beijing Zhongyan Tongrentang Pharmaceutical Research and Development Co. Ltd.

Beijing Tongrentang Science and Technology Development Co. Ltd.

Department of Pharmacy,Jiangxi University of Traditional Chinese Medicine

Guangdong Pharmaceutical University

Sun Yat-sen University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰