Objective: To study the effects of Oviductus Ranae on the content of testosterone

substance metabolism and anti-fatigue ability of rats after exercise. Method: By using the model of high-intensity endurance training

fifty-five male Wistar rats were randomly divided into 5 groups

with 10 in each group. The rats which did not meet the requirement were removed:still in control group (C group)

motion control group (M group)

exercise +ig low-dose Oviductus Ranae group (OML group)

exercise+ig middle-dose Oviductus Ranae group (OMM group)

and exercise+ig high-dose Oviductus Ranae group (OMH group). Gavage was performed using professional device once a day. The rats in Oviductus Ranae groups were gavaged with 0.5

1.0

3.0 g·kg-1 with ig volume of 5 mL·kg-1. The rats in C and M groups were given saline of same volume. After 42 days of exhaustive swimming training

body weight

swimming time and serum testosterone and other biochemical markers were measured. Result: The body weights of the rats in M groups were lower than those in C group (P<0.05)

and those in all doses of Oviductus Ranae groups were higher than in M group (P<0.05) which showed no differences between groups. Swimming time in all doses of Oviductus Ranae groups was longer than M group (P<0.01)with a dose-effect relationship

But there were no differences between C and M groups. Serum testosterone in M group was lower than C group (P<0.01)

in the same time

serum testosterone was higher in all doses of Oviductus Ranae groups than M group [OML:(4.96±1.60) nmol·L-1;OMM:(5.19±1.60) nmol·L-1;OMH:(5.35±1.66) nmol·L-1

P<0.01]. The serum corticosterone levels in each group showed no significant differences. Changes in the ratio of serum testosterone/corticosterone were more consistent with testosterone changes among the groups. Liver glycogen and muscle glycogen (P<0.01) in M group were lower than in C group

and in all doses of Oviductus Ranae groups were higher than in M group (liver glycogen

P<0.05;muscle glycogen

P<0.01). There were no differences in luteinizing hormone and follicle-stimulating hormone between groups. The serum urea nitrogen in M group was higher than C group (P<0.01)

and in all doses of Oviductus Ranae groups was lower than the M group (P<0.01). Hemoglobin in M group was lower than C group (P<0.01)

and in all doses of Oviductus Ranae groups were higher than in M group(P<0.01). Conclusion: Oviductus Ranae can alleviate the impact of high-intensity exercise on serum testosterone

and maintained it at normal physiological levels;it can also promote protein synthesis

inhibit degradation of amino acid and protein

and increase hemoglobin and glycogen reserves in rats exercise training.