Influence of Meth-ferulic Acid on Proliferation and Expression of -smooth Muscle Actin(-SMA) in TGF--induced Human Hepatic Stellate Cells

CHENG Yu; CHEN Hong-zhen

doi:10.13422/j.cnki.syfjx.2014200164

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Influence of Meth-ferulic Acid on Proliferation and Expression of -smooth Muscle Actin(-SMA) in TGF--induced Human Hepatic Stellate Cells

更新时间：2024-01-04

- Influence of Meth-ferulic Acid on Proliferation and Expression of -smooth Muscle Actin(-SMA) in TGF--induced Human Hepatic Stellate Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 20, Pages: 164-167(2014)
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- DOI：10.13422/j.cnki.syfjx.2014200164
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- Published：2014
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CHENG Yu, CHEN Hong-zhen. Influence of Meth-ferulic Acid on Proliferation and Expression of -smooth Muscle Actin(-SMA) in TGF--induced Human Hepatic Stellate Cells[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 164-167.
DOI：

CHENG Yu, CHEN Hong-zhen. Influence of Meth-ferulic Acid on Proliferation and Expression of -smooth Muscle Actin(-SMA) in TGF--induced Human Hepatic Stellate Cells[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 164-167. DOI： 10.13422/j.cnki.syfjx.2014200164.

摘要

目的：研究甲基阿魏酸（MFA）对转化生长因子-β1（TGF-β1）刺激的人肝星状细胞（HSC-LX2）增殖与α-平滑肌激动蛋白（α-SMA）表达的影响。方法：将5×107个/L HSC-LX2进行体外培养，以不同剂量MFA（终质量浓度为5，10，20 mg ·L-1）干预TGF-β1刺激的HSC-LX2 48 h，用MTT法检测MFA对TGF-β1刺激的HSC-LX2细胞增殖的影响；应用RT-PCR方法及Western blotting 技术检测对 HSC-LX2的α-SMA表达的影响。结果：MFA可抑制TGF-β1诱导的HSC-LX2的增殖，以5 mg ·L-1时抑制作用最为明显，与TGF-β1诱导的模型组比较有显著性差异（P<0.05）；与模型组比较，MFA各剂量组作用于HSC-LX2 48 h后，HSC-LX2细胞中α-SMA mRNA与蛋白表达的水平降低，且5 mg ·L-1时作用最明显（P<0.05）。结论：MFA预处理人肝星状细胞可下调α-SMA的mRNA及蛋白水平，从而推断MFA可以抑制HSC增殖及活化，抑制肝纤维化的发生和发展。

Abstract

Objective: To investigate influence of meth-ferulic acid (MFA) on the proliferation and expression of α-smooth muscle actin (α-SMA) in transforming growth factor beta 1(TGF-β1)-induced human hepatic stellate cells (HSC-LX2). Method: TGF-β1 5×107/L induced human hepatic stellate cell line (HSC-LX2) in vitro were cultured with different dose of MFA(the final concentration being 5

20 mg·L-1). MTT method was used to detect the proliferation of TGF-β1-induced HSC-LX2. Western blotting and RT-PCR were used to detect the expression of α-smooth muscle actin (α-SMA) of HSC-LX2. Result: MFA could inhibit the proliferation of TGF-β1-induced HSC-LX2 with a dose dependent

the inhibitory effect by 5 mg·L-1 concentration was more obvious

compared with the TGF-β1-induced the model group(P<0.05);and compared with TGF-β1-induced the model group

the expression of α-SMA mRNA and protein by 5 mg·L-1concentration was more obvious. Conclusion: MFA pretreatment on human hepatic stellate cells can reduce α -SMA mRNA and protein levels.

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